Search Results

Overview

Uniprot IDP11766
Protein NameAlcohol dehydrogenase class-3
Gene NameADH5
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
107 CKFCLNPKTNLCQKI
113 PKTNLCQKIRVTQGK
120 KIRVTQGKGLMPDGT
226 IIGVDINKDKFARAK
233 KDKFARAKEFGATEC
284 AALEACHKGWGVSVV
315 LVTGRTWKGTAFGGW
323 GTAFGGWKSVESVPK
338 LVSEYMSKKIKVDEF
366 FELMHSGKSIRTVVK
84 GEGVTKLKAGDTVIP

Function

Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione (PubMed:8460164). Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate (PubMed:16081420). Class-III ADH is remarkably ineffective in oxidizing ethanol (PubMed:8460164). Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage (PubMed:33355142). Also acts as a S-nitroso-glutathione reductase by catalyzing the NADH-dependent reduction of S-nitrosoglutathione, thereby regulating protein S-nitrosylation (By similarity)

Protein Sequence

10 MANEVIKCKA 20 AVAWEAGKPL 30 SIEEIEVAPP 40 KAHEVRIKII 50 ATAVCHTDAY 60 TLSGADPEGC 70 FPVILGHEGA 80 GIVESVGEGV 90 TKLKAGDTVI 100 PLYIPQCGEC 110 KFCLNPKTNL 120 CQKIRVTQGK 130 GLMPDGTSRF 140 TCKGKTILHY 150 MGTSTFSEYT 160 VVADISVAKI 170 DPLAPLDKVC 180 LLGCGISTGY 190 GAAVNTAKLE 200 PGSVCAVFGL 210 GGVGLAVIMG 220 CKVAGASRII 230 GVDINKDKFA 240 RAKEFGATEC 250 INPQDFSKPI 260 QEVLIEMTDG 270 GVDYSFECIG 280 NVKVMRAALE 290 ACHKGWGVSV 300 VVGVAASGEE 310 IATRPFQLVT 320 GRTWKGTAFG 330 GWKSVESVPK 340 LVSEYMSKKI 350 KVDEFVTHNL 360 SFDEINKAFE 370 LMHSGKSIRT VVKI

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Molecular Function GO:0004022 alcohol dehydrogenase (NAD+) activity
Molecular Function GO:0009055 electron transfer activity
Molecular Function GO:0005504 fatty acid binding
Molecular Function GO:0018467 formaldehyde dehydrogenase (NAD+) activity
Molecular Function GO:0106322 S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity
Molecular Function GO:0106321 S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity
Molecular Function GO:0051903 S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity
Molecular Function GO:0080007 S-nitrosoglutathione reductase (NADH) activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0010430 fatty acid omega-oxidation
Biological Process GO:0046294 formaldehyde catabolic process
Biological Process GO:0051775 response to redox state

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.