Search Results

Overview

Uniprot IDP11980
Protein NamePyruvate kinase PKM
Gene NamePkm
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
115 VAVALDTKGPEIRTG
125 EIRTGLIKGSGTAEV
135 GTAEVELKKGATLKI
141 LKKGATLKITLDNAY
166 LDYKNICKVVEVGSK
207 GGSLGSKKGVNLPGA
247 VFASFIRKAADVHEV
261 VRKVLGEKGKNIKII
270 KNIKIISKIENHEGV
305 GIEIPAEKVFLAQKM
322 GRCNRAGKPVICATQ
498 NLAMNVGKARGFFKK
62 SRSVEMLKEMIKSGM
66 EMLKEMIKSGMNVAR
89 EYHAETIKNVRAATE

Function

Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival

Protein Sequence

10 MPKPDSEAGT 20 AFIQTQQLHA 30 AMADTFLEHM 40 CRLDIDSAPI 50 TARNTGIICT 60 IGPASRSVEM 70 LKEMIKSGMN 80 VARLNFSHGT 90 HEYHAETIKN 100 VRAATESFAS 110 DPILYRPVAV 120 ALDTKGPEIR 130 TGLIKGSGTA 140 EVELKKGATL 150 KITLDNAYME 160 KCDENILWLD 170 YKNICKVVEV 180 GSKIYVDDGL 190 ISLQVKEKGA 200 DYLVTEVENG 210 GSLGSKKGVN 220 LPGAAVDLPA 230 VSEKDIQDLK 240 FGVEQDVDMV 250 FASFIRKAAD 260 VHEVRKVLGE 270 KGKNIKIISK 280 IENHEGVRRF 290 DEILEASDGI 300 MVARGDLGIE 310 IPAEKVFLAQ 320 KMMIGRCNRA 330 GKPVICATQM 340 LESMIKKPRP 350 TRAEGSDVAN 360 AVLDGADCIM 370 LSGETAKGDY 380 PLEAVRMQHL 390 IAREAEAAVF 400 HRLLFEELAR 410 ASSQSTDPLE 420 AMAMGSVEAS 430 YKCLAAALIV 440 LTESGRSAHQ 450 VARYRPRAPI 460 IAVTRNPQTA 470 RQAHLYRGIF 480 PVLCKDAVLD 490 AWAEDVDLRV 500 NLAMNVGKAR 510 GFFKKGDVVI 520 VLTGWRPGSG 530 FTNTMRVVPV P

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005929 cilium
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005634 nucleus
Cellular Component GO:0001917 photoreceptor inner segment
Cellular Component GO:1902912 pyruvate kinase complex
Cellular Component GO:0005791 rough endoplasmic reticulum
Molecular Function GO:0043531 ADP binding
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0035402 histone H3T11 kinase activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0000287 magnesium ion binding
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0004715 non-membrane spanning protein tyrosine kinase activity
Molecular Function GO:0030955 potassium ion binding
Molecular Function GO:0046983 protein dimerization activity
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0004713 protein tyrosine kinase activity
Molecular Function GO:0004743 pyruvate kinase activity
Molecular Function GO:0070324 thyroid hormone binding
Molecular Function GO:0003713 transcription coactivator activity
Biological Process GO:0031100 animal organ regeneration
Biological Process GO:0061621 canonical glycolysis
Biological Process GO:0032869 cellular response to insulin stimulus
Biological Process GO:0006006 glucose metabolic process
Biological Process GO:0006096 glycolytic process
Biological Process GO:0001889 liver development
Biological Process GO:2000767 positive regulation of cytoplasmic translation
Biological Process GO:1903672 positive regulation of sprouting angiogenesis
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0012501 programmed cell death
Biological Process GO:0051289 protein homotetramerization
Biological Process GO:0051262 protein tetramerization
Biological Process GO:0042866 pyruvate biosynthetic process
Biological Process GO:0009629 response to gravity
Biological Process GO:0001666 response to hypoxia
Biological Process GO:0032868 response to insulin
Biological Process GO:0014870 response to muscle inactivity
Biological Process GO:0007584 response to nutrient
Biological Process GO:0043403 skeletal muscle tissue regeneration

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[3] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.