Search Results

Overview

Uniprot IDP12270
Protein NameNucleoprotein TPR
Gene NameTPR
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1081 IAVEAQNKYERELML
1379 TEEIGRLKAEIARSN
1442 KKIGRRYKTQYEELK
1561 RKAIVAAKSKIAHLA
1563 AIVAAKSKIAHLAGV
1603 DVRITALKSQYEGRI
1648 QQRQITLKTTPASGE
1680 PVVSTPSKVTAAAMA
1690 AAAMAGNKSTPRASI
1829 TPSSSLPKRTREEEE
364 NDLLSATKRKGAILS
387 PTAAAVAKIVKPGMK
852 EHEISHLKKKLENEV
854 EISHLKKKLENEVEQ
877 DVQLLDTKRQLDTET
925 QSSQRTGKGQPSNKE

Function

Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs, plays a role in the establishment of nuclear-peripheral chromatin compartmentalization in interphase, and in the mitotic spindle checkpoint signaling during mitosis. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with NUP153, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Negatively regulates both the association of CTE-containing mRNA with large polyribosomes and translation initiation. Does not play any role in Rev response element (RRE)-mediated export of unspliced mRNAs. Implicated in nuclear export of mRNAs transcribed from heat shock gene promoters; associates both with chromatin in the HSP70 promoter and with mRNAs transcribed from this promoter under stress-induced conditions. Modulates the nucleocytoplasmic transport of activated MAPK1/ERK2 and huntingtin/HTT and may serve as a docking site for the XPO1/CRM1-mediated nuclear export complex. According to some authors, plays a limited role in the regulation of nuclear protein export (PubMed:11952838, PubMed:22253824). Also plays a role as a structural and functional element of the perinuclear chromatin distribution; involved in the formation and/or maintenance of NPC-associated perinuclear heterochromatin exclusion zones (HEZs). Finally, acts as a spatial regulator of the spindle-assembly checkpoint (SAC) response ensuring a timely and effective recruitment of spindle checkpoint proteins like MAD1L1 and MAD2L1 to unattached kinetochore during the metaphase-anaphase transition before chromosome congression. Its N-terminus is involved in activation of oncogenic kinases

Protein Sequence

10 MAAVLQQVLE 20 RTELNKLPKS 30 VQNKLEKFLA 40 DQQSEIDGLK 50 GRHEKFKVES 60 EQQYFEIEKR 70 LSHSQERLVN 80 ETRECQSLRL 90 ELEKLNNQLK 100 ALTEKNKELE 110 IAQDRNIAIQ 120 SQFTRTKEEL 130 EAEKRDLIRT 140 NERLSQELEY 150 LTEDVKRLNE 160 KLKESNTTKG 170 ELQLKLDELQ 180 ASDVSVKYRE 190 KRLEQEKELL 200 HSQNTWLNTE 210 LKTKTDELLA 220 LGREKGNEIL 230 ELKCNLENKK 240 EEVSRLEEQM 250 NGLKTSNEHL 260 QKHVEDLLTK 270 LKEAKEQQAS 280 MEEKFHNELN 290 AHIKLSNLYK 300 SAADDSEAKS 310 NELTRAVEEL 320 HKLLKEAGEA 330 NKAIQDHLLE 340 VEQSKDQMEK 350 EMLEKIGRLE 360 KELENANDLL 370 SATKRKGAIL 380 SEEELAAMSP 390 TAAAVAKIVK 400 PGMKLTELYN 410 AYVETQDQLL 420 LEKLENKRIN 430 KYLDEIVKEV 440 EAKAPILKRQ 450 REEYERAQKA 460 VASLSVKLEQ 470 AMKEIQRLQE 480 DTDKANKQSS 490 VLERDNRRME 500 IQVKDLSQQI 510 RVLLMELEEA 520 RGNHVIRDEE 530 VSSADISSSS 540 EVISQHLVSY 550 RNIEELQQQN 560 QRLLVALREL 570 GETREREEQE 580 TTSSKITELQ 590 LKLESALTEL 600 EQLRKSRQHQ 610 MQLVDSIVRQ 620 RDMYRILLSQ 630 TTGVAIPLHA 640 SSLDDVSLAS 650 TPKRPSTSQT 660 VSTPAPVPVI 670 ESTEAIEAKA 680 ALKQLQEIFE 690 NYKKEKAENE 700 KIQNEQLEKL 710 QEQVTDLRSQ 720 NTKISTQLDF 730 ASKRYEMLQD 740 NVEGYRREIT 750 SLHERNQKLT 760 ATTQKQEQII 770 NTMTQDLRGA 780 NEKLAVAEVR 790 AENLKKEKEM 800 LKLSEVRLSQ 810 QRESLLAEQR 820 GQNLLLTNLQ 830 TIQGILERSE 840 TETKQRLSSQ 850 IEKLEHEISH 860 LKKKLENEVE 870 QRHTLTRNLD 880 VQLLDTKRQL 890 DTETNLHLNT 900 KELLKNAQKE 910 IATLKQHLSN 920 MEVQVASQSS 930 QRTGKGQPSN 940 KEDVDDLVSQ 950 LRQTEEQVND 960 LKERLKTSTS 970 NVEQYQAMVT 980 SLEESLNKEK 990 QVTEEVRKNI 1000 EVRLKESAEF 1010 QTQLEKKLME 1020 VEKEKQELQD 1030 DKRRAIESME 1040 QQLSELKKTL 1050 SSVQNEVQEA 1060 LQRASTALSN 1070 EQQARRDCQE 1080 QAKIAVEAQN 1090 KYERELMLHA 1100 ADVEALQAAK 1110 EQVSKMASVR 1120 QHLEETTQKA 1130 ESQLLECKAS 1140 WEERERMLKD 1150 EVSKCVCRCE 1160 DLEKQNRLLH 1170 DQIEKLSDKV 1180 VASVKEGVQG 1190 PLNVSLSEEG 1200 KSQEQILEIL 1210 RFIRREKEIA 1220 ETRFEVAQVE 1230 SLRYRQRVEL 1240 LERELQELQD 1250 SLNAEREKVQ 1260 VTAKTMAQHE 1270 ELMKKTETMN 1280 VVMETNKMLR 1290 EEKERLEQDL 1300 QQMQAKVRKL 1310 ELDILPLQEA 1320 NAELSEKSGM 1330 LQAEKKLLEE 1340 DVKRWKARNQ 1350 HLVSQQKDPD 1360 TEEYRKLLSE 1370 KEVHTKRIQQ 1380 LTEEIGRLKA 1390 EIARSNASLT 1400 NNQNLIQSLK 1410 EDLNKVRTEK 1420 ETIQKDLDAK 1430 IIDIQEKVKT 1440 ITQVKKIGRR 1450 YKTQYEELKA 1460 QQDKVMETSA 1470 QSSGDHQEQH 1480 VSVQEMQELK 1490 ETLNQAETKS 1500 KSLESQVENL 1510 QKTLSEKETE 1520 ARNLQEQTVQ 1530 LQSELSRLRQ 1540 DLQDRTTQEE 1550 QLRQQITEKE 1560 EKTRKAIVAA 1570 KSKIAHLAGV 1580 KDQLTKENEE 1590 LKQRNGALDQ 1600 QKDELDVRIT 1610 ALKSQYEGRI 1620 SRLERELREH 1630 QERHLEQRDE 1640 PQEPSNKVPE 1650 QQRQITLKTT 1660 PASGERGIAS 1670 TSDPPTANIK 1680 PTPVVSTPSK 1690 VTAAAMAGNK 1700 STPRASIRPM 1710 VTPATVTNPT 1720 TTPTATVMPT 1730 TQVESQEAMQ 1740 SEGPVEHVPV 1750 FGSTSGSVRS 1760 TSPNVQPSIS 1770 QPILTVQQQT 1780 QATAFVQPTQ 1790 QSHPQIEPAN 1800 QELSSNIVEV 1810 VQSSPVERPS 1820 TSTAVFGTVS 1830 ATPSSSLPKR 1840 TREEEEDSTI 1850 EASDQVSDDT 1860 VEMPLPKKLK 1870 SVTPVGTEEE 1880 VMAEESTDGE 1890 VETQVYNQDS 1900 QDSIGEGVTQ 1910 GDYTPMEDSE 1920 ETSQSLQIDL 1930 GPLQSDQQTT 1940 TSSQDGQGKG 1950 DDVIVIDSDD 1960 EEEDDDENDG 1970 EHEDYEEDEE 1980 DDDDDEDDTG 1990 MGDEGEDSNE 2000 GTGSADGNDG 2010 YEADDAEGGD 2020 GTDPGTETEE 2030 SMGGGEGNHR 2040 AADSQNSGEG 2050 NTGAAESSFS 2060 QEVSREQQPS 2070 SASERQAPRA 2080 PQSPRRPPHP 2090 LPPRLTIHAP 2100 PQELGPPVQR 2110 IQMTRRQSVG 2120 RGLQLTPGIG 2130 GMQQHFFDDE 2140 DRTVPSTPTL 2150 VVPHRTDGFA 2160 EAIHSPQVAG 2170 VPRFRFGPPE 2180 DMPQTSSSHS 2190 DLGQLASQGG 2200 LGMYETPLFL 2210 AHEEESGGRS 2220 VPTTPLQVAA 2230 PVTVFTESTT 2240 SDASEHASQS 2250 VPMVTTSTGT 2260 LSTTNETATG 2270 DDGDEVFVEA 2280 ESEGISSEAG 2290 LEIDSQQEEE 2300 PVQASDESDL 2310 PSTSQDPPSS 2320 SSVDTSSSQP 2330 KPFRRVRLQT 2340 TLRQGVRGRQ 2350 FNRQRGVSHA 2360 MGGRGGINRG NIN

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005868 cytoplasmic dynein complex
Cellular Component GO:0000776 kinetochore
Cellular Component GO:0072686 mitotic spindle
Cellular Component GO:0005635 nuclear envelope
Cellular Component GO:0042405 nuclear inclusion body
Cellular Component GO:0031965 nuclear membrane
Cellular Component GO:0034399 nuclear periphery
Cellular Component GO:0005643 nuclear pore
Cellular Component GO:0044615 nuclear pore nuclear basket
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0070840 dynein complex binding
Molecular Function GO:0031072 heat shock protein binding
Molecular Function GO:0051019 mitogen-activated protein kinase binding
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0017056 structural constituent of nuclear pore
Molecular Function GO:0015631 tubulin binding
Biological Process GO:0051301 cell division
Biological Process GO:0034605 cellular response to heat
Biological Process GO:0035457 cellular response to interferon-alpha
Biological Process GO:0007094 mitotic spindle assembly checkpoint signaling
Biological Process GO:0006406 mRNA export from nucleus
Biological Process GO:0031990 mRNA export from nucleus in response to heat stress
Biological Process GO:0046832 negative regulation of RNA export from nucleus
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0045947 negative regulation of translational initiation
Biological Process GO:0006999 nuclear pore organization
Biological Process GO:0006913 nucleocytoplasmic transport
Biological Process GO:0031453 positive regulation of heterochromatin formation
Biological Process GO:0090316 positive regulation of intracellular protein transport
Biological Process GO:0090267 positive regulation of mitotic cell cycle spindle assembly checkpoint
Biological Process GO:0046827 positive regulation of protein export from nucleus
Biological Process GO:0042307 positive regulation of protein import into nucleus
Biological Process GO:0006606 protein import into nucleus
Biological Process GO:0010965 regulation of mitotic sister chromatid separation
Biological Process GO:1901673 regulation of mitotic spindle assembly
Biological Process GO:0032880 regulation of protein localization
Biological Process GO:0070849 response to epidermal growth factor
Biological Process GO:0006405 RNA export from nucleus
Biological Process GO:0006404 RNA import into nucleus

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[4] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[5] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[6] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[7] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[8] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.