Search Results

Overview

Uniprot IDP12277
Protein NameCreatine kinase B-type
Gene NameCKB
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
11 SNSHNALKLRFPAED
156 GERRAIEKLAVEALS
242 LRVISMQKGGNMKEV
304 IKLPNLGKHEKFSEV
307 PNLGKHEKFSEVLKR
313 EKFSEVLKRLRLQKR
32 AHNNHMAKVLTPELY

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed:8186255). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation (By similarity). During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work (By similarity)

Protein Sequence

10 MPFSNSHNAL 20 KLRFPAEDEF 30 PDLSAHNNHM 40 AKVLTPELYA 50 ELRAKSTPSG 60 FTLDDVIQTG 70 VDNPGHPYIM 80 TVGCVAGDEE 90 SYEVFKDLFD 100 PIIEDRHGGY 110 KPSDEHKTDL 120 NPDNLQGGDD 130 LDPNYVLSSR 140 VRTGRSIRGF 150 CLPPHCSRGE 160 RRAIEKLAVE 170 ALSSLDGDLA 180 GRYYALKSMT 190 EAEQQQLIDD 200 HFLFDKPVSP 210 LLLASGMARD 220 WPDARGIWHN 230 DNKTFLVWVN 240 EEDHLRVISM 250 QKGGNMKEVF 260 TRFCTGLTQI 270 ETLFKSKDYE 280 FMWNPHLGYI 290 LTCPSNLGTG 300 LRAGVHIKLP 310 NLGKHEKFSE 320 VLKRLRLQKR 330 GTGGVDTAAV 340 GGVFDVSNAD 350 RLGFSEVELV 360 QMVVDGVKLL 370 IEMEQRLEQG 380 QAIDDLMPAQ K

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005615 extracellular space
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005886 plasma membrane
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004111 creatine kinase activity
Molecular Function GO:0031625 ubiquitin protein ligase binding
Biological Process GO:0140651 futile creatine cycle
Biological Process GO:0046314 phosphocreatine biosynthetic process
Biological Process GO:0021762 substantia nigra development

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.