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Overview

Uniprot ID	P12787
Protein Name	Cytochrome c oxidase subunit 5A, mitochondrial
Gene Name	Cox5a
Organism	Mus musculus

Kla Sites from experimental identification

Position	Flanking peptide
109	VRILEVVKDKAGPHK

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix

Protein Sequence

10 MLAAALRRCT 20 AAAAARGLLH 30 PASAPSPAAA 40 VCSIRCYSHG 50 SHETDEEFDA 60 RWVTYFNKPD 70 IDAWELRKGM 80 NTLVGYDLVP 90 EPKIIDAALR 100 ACRRLNDFAS 110 AVRILEVVKD 120 KAGPHKEIYP 130 YVIQELRPTL 140 NELGISTPEE LGLDKV

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005743	mitochondrial inner membrane
Cellular Component	GO:0031966	mitochondrial membrane
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0043209	myelin sheath
Cellular Component	GO:0045277	respiratory chain complex IV
Molecular Function	GO:0004129	cytochrome-c oxidase activity
Molecular Function	GO:0046872	metal ion binding
Biological Process	GO:0006123	mitochondrial electron transport, cytochrome c to oxygen

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.