Overview
| Uniprot ID | P13437 |
|---|
| Protein Name | 3-ketoacyl-CoA thiolase, mitochondrial |
|---|
| Gene Name | Acaa2 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 214 |
EVKTKKGKQTMQVDE |
Function
In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (Probable). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (Probable). Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies (Probable). Also displays hydrolase activity on various fatty acyl-CoAs (PubMed:16476568). Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation (Probable). Abolishes BNIP3-mediated apoptosis and mitochondrial damage (By similarity)
Protein Sequence
10
MALLRGVFIV
20
AAKRTPFGAY
30
GGLLKDFTAT
40
DLTEFAARAA
50
LSAGKVPPET
60
IDSVIVGNVM
70
QSSSDAAYLA
80
RHVGLRVGVP
90
TETGALTLNR
100
LCGSGFQSIV
110
SGCQEICSKD
120
AEVVLCGGTE
130
SMSQSPYSVR
140
NVRFGTKFGL
150
DLKLEDTLWA
160
GLTDQHVKLP
170
MGMTAENLAA
180
KYNISREDCD
190
RYALQSQQRW
200
KAANEAGYFN
210
EEMAPIEVKT
220
KKGKQTMQVD
230
EHARPQTTLE
240
QLQNLPPVFK
250
KEGTVTAGNA
260
SGMSDGAGVV
270
IIASEDAVKK
280
HNFTPLARVV
290
GYFVSGCDPA
300
IMGIGPVPAI
310
TGALKKAGLS
320
LKDMDLIDVN
330
EAFAPQFLAV
340
QKSLDLDPSK
350
TNVSGGAIAL
360
GHPLGGSGSR
370
ITAHLVHELR
380
RRGGKYAVGS
390
ACIGGGQGIS
LIIQNTA
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005759 |
mitochondrial matrix |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Molecular Function |
GO:0003985 |
acetyl-CoA C-acetyltransferase activity |
| Molecular Function |
GO:0003988 |
acetyl-CoA C-acyltransferase activity |
| Molecular Function |
GO:0003986 |
acetyl-CoA hydrolase activity |
| Molecular Function |
GO:0047617 |
fatty acyl-CoA hydrolase activity |
| Biological Process |
GO:0006084 |
acetyl-CoA metabolic process |
| Biological Process |
GO:0071456 |
cellular response to hypoxia |
| Biological Process |
GO:0006635 |
fatty acid beta-oxidation |
| Biological Process |
GO:1902109 |
negative regulation of mitochondrial membrane permeability involved in apoptotic process |
| Biological Process |
GO:1901029 |
negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.