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Overview

Uniprot IDP14604
Protein NameEnoyl-CoA hydratase, mitochondrial
Gene NameEchs1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
101 FAAGADIKEMQNRTF
204 RISAQDAKQAGLVSK
43 IITEKKGKNSSVGLI

Function

Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl-CoA) to the corresponding 3(S)-3-hydroxyacyl-CoA species through addition of a water molecule to the double bond (PubMed:10074351, PubMed:7883013). Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16 (PubMed:10074351, PubMed:7883013). Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA) and methacrylyl-CoA ((2E)-2-methylpropenoyl-CoA). Can bind tiglyl-CoA (2-methylcrotonoyl-CoA), but hydrates only a small amount of this substrate (By similarity). Plays a key role in the beta-oxidation spiral of short- and medium-chain fatty acid oxidation (PubMed:7883013). At a lower rate than the hydratase reaction, catalyzes the isomerase reaction of trans-3-enoyl-CoA species (such as (3E)-hexenoyl-CoA) to trans-2-enoyl-CoA species (such as (2E)-hexenoyl-CoA), which are subsequently hydrated to 3(S)-3-hydroxyacyl-CoA species (such as (3S)-hydroxyhexanoyl-CoA) (PubMed:10074351)

Protein Sequence

10 MAALRALLPR 20 ACNSLLSPVR 30 CPEFRRFASG 40 ANFQYIITEK 50 KGKNSSVGLI 60 QLNRPKALNA 70 LCNGLIEELN 80 QALETFEEDP 90 AVGAIVLTGG 100 EKAFAAGADI 110 KEMQNRTFQD 120 CYSGKFLSHW 130 DHITRIKKPV 140 IAAVNGYALG 150 GGCELAMMCD 160 IIYAGEKAQF 170 GQPEILLGTI 180 PGAGGTQRLT 190 RAVGKSLAME 200 MVLTGDRISA 210 QDAKQAGLVS 220 KIFPVETLVE 230 EAIQCAEKIA 240 NNSKIIVAMA 250 KESVNAAFEM 260 TLTEGNKLEK 270 KLFYSTFATD 280 DRREGMSAFV 290 EKRKANFKDH

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0120092 (2E)-butenoyl-CoA hydratase activity
Molecular Function GO:0043956 3-hydroxypropionyl-CoA dehydratase activity
Molecular Function GO:0004165 delta(3)-delta(2)-enoyl-CoA isomerase activity
Molecular Function GO:0004300 enoyl-CoA hydratase activity
Biological Process GO:0006635 fatty acid beta-oxidation
Biological Process GO:0006574 L-valine catabolic process

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.