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Overview

Uniprot ID	P14659
Protein Name	Heat shock-related 70 kDa protein 2
Gene Name	Hspa2
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
72	TNTIFDAKRLIGRKF

Function

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. Plays a role in spermatogenesis. In association with SHCBP1L may participate in the maintenance of spindle integrity during meiosis in male germ cells

Protein Sequence

10 MSARGPAIGI 20 DLGTTYSCVG 30 VFQHGKVEII 40 ANDQGNRTTP 50 SYVAFTDTER 60 LIGDAAKNQV 70 AMNPTNTIFD 80 AKRLIGRKFE 90 DATVQSDMKH 100 WPFRVVSEGG 110 KPKVQVEYKG 120 EMKTFFPEEI 130 SSMVLTKMKE 140 IAEAYLGGKV 150 QSAVITVPAY 160 FNDSQRQATK 170 DAGTITGLNV 180 LRIINEPTAA 190 AIAYGLDKKG 200 CAGGEKNVLI 210 FDLGGGTFDV 220 SILTIEDGIF 230 EVKSTAGDTH 240 LGGEDFDNRM 250 VSHLAEEFKR 260 KHKKDIGPNK 270 RAVRRLRTAC 280 ERAKRTLSSS 290 TQASIEIDSL 300 YEGVDFYTSI 310 TRARFEELNA 320 DLFRGTLEPV 330 EKALRDAKLD 340 KGQIQEIVLV 350 GGSTRIPKIQ 360 KLLQDFFNGK 370 ELNKSINPDE 380 AVAYGAAVQA 390 AILIGDKSEN 400 VQDLLLLDVT 410 PLSLGIETAG 420 GVMTPLIKRN 430 TTIPTKQTQT 440 FTTYSDNQSS 450 VLVQVYEGER 460 AMTKDNNLLG 470 KFDLTGIPPA 480 PRGVPQIEVT 490 FDIDANGILN 500 VTAADKSTGK 510 ENKITITNDK 520 GRLSKDDIDR 530 MVQEAERYKS 540 EDEANRDRVA 550 AKNAVESYTY 560 NIKQTVEDEK 570 LRGKISEQDK 580 NKILDKCQEV 590 INWLDRNQMA 600 EKDEYEHKQK 610 ELERVCNPII 620 SKLYQGGPGG 630 GGSSGGPTIE EVD

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0036128	CatSper complex
Cellular Component	GO:0009986	cell surface
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0001673	male germ cell nucleus
Cellular Component	GO:0072687	meiotic spindle
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0005886	plasma membrane
Cellular Component	GO:0000795	synaptonemal complex
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0016887	ATP hydrolysis activity
Molecular Function	GO:0097718	disordered domain specific binding
Molecular Function	GO:0019899	enzyme binding
Molecular Function	GO:0051861	glycolipid binding
Molecular Function	GO:0031072	heat shock protein binding
Molecular Function	GO:0044183	protein folding chaperone
Molecular Function	GO:0051087	protein-folding chaperone binding
Molecular Function	GO:0048156	tau protein binding
Molecular Function	GO:0051082	unfolded protein binding
Biological Process	GO:0007141	male meiosis I
Biological Process	GO:0007140	male meiotic nuclear division
Biological Process	GO:0090084	negative regulation of inclusion body assembly
Biological Process	GO:0010971	positive regulation of G2/M transition of mitotic cell cycle
Biological Process	GO:0001934	positive regulation of protein phosphorylation
Biological Process	GO:0042026	protein refolding
Biological Process	GO:0009409	response to cold
Biological Process	GO:0009408	response to heat
Biological Process	GO:0007286	spermatid development
Biological Process	GO:0007283	spermatogenesis
Biological Process	GO:0070194	synaptonemal complex disassembly

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.