Search Results
Overview
| Uniprot ID | P15531 |
|---|---|
| Protein Name | Nucleoside diphosphate kinase A |
| Gene Name | NME1 |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 100 | ETNPADSKPGTIRGD |
| 12 | ERTFIAIKPDGVQRG |
| 26 | GLVGEIIKRFEQKGF |
| 31 | IIKRFEQKGFRLVGL |
| 49 | QASEDLLKEHYVDLK |
Function
Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefore contributing to the nucleoside triphosphate homeostasis (PubMed:10952986, PubMed:14960567, PubMed:16313181, PubMed:1851158, PubMed:23519676, PubMed:33903070, PubMed:8810265, PubMed:9038158). Also phosphorylates geranyl pyrophosphate (GPP) and farnesyl pyrophosphate (FPP), linking it to isoprenoid metabolism (PubMed:10952986). Additionally, functions as a non-specific serine/threonine kinase and histidine protein kinase, transferring phosphoryl groups from its active site to target proteins (PubMed:8529641, PubMed:9038158). May function as a Mg(2+)-dependent single-stranded DNA endonuclease as part of the SET complex, cooperating with the 3'-5' exonuclease TREX1 to mediate apoptotic DNA fragmentation in cytotoxic T lymphocytes (PubMed:12628186, PubMed:16818237). Reported to nick one DNA strand, enabling TREX1 to remove nucleotides from the free 3' end, enhancing DNA damage and suppressing DNA end reannealing and repair (PubMed:16818237). Has been shown to cleave double strands DNA within the 3'-portions of both 5'-SHS silencer and NHE basal promoter element of the PDGFA gene, potentially repressing its transcription (PubMed:11694515). May also function as a Mg(2+)-dependent 3'-5' DNA exonuclease, excising nucleotides from 3' single-stranded DNA or DNA with 3' single strand overhangs, suggesting a role in DNA nucleolytic processing (PubMed:14960567, PubMed:16313181). Involved in the regulation of tumor metastasis and cellular differentiation (By similarity). Also required for cell motility (PubMed:8270257, PubMed:25582197). May control, with NME2, AcCoA usage between histone acetylation and fatty acid synthesis, possibly by binding and releasing AcCoA at transcriptionally active chromatin regions in proximity to histone acetyltransferase (HAT) (By similarity)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Biological Process | GO:0007399 | nervous system development |
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0005829 | cytosol |
| Cellular Component | GO:0005769 | early endosome |
| Cellular Component | GO:0070062 | extracellular exosome |
| Cellular Component | GO:0016020 | membrane |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0032587 | ruffle membrane |
| Molecular Function | GO:0008296 | 3'-5'-DNA exonuclease activity |
| Molecular Function | GO:1905502 | acetyl-CoA binding |
| Molecular Function | GO:0043531 | ADP binding |
| Molecular Function | GO:0005524 | ATP binding |
| Molecular Function | GO:0120225 | coenzyme A binding |
| Molecular Function | GO:0003677 | DNA binding |
| Molecular Function | GO:0004520 | DNA endonuclease activity |
| Molecular Function | GO:0004536 | DNA nuclease activity |
| Molecular Function | GO:0047887 | farnesyl diphosphate kinase activity |
| Molecular Function | GO:0019003 | GDP binding |
| Molecular Function | GO:0042802 | identical protein binding |
| Molecular Function | GO:0016301 | kinase activity |
| Molecular Function | GO:0000287 | magnesium ion binding |
| Molecular Function | GO:0004550 | nucleoside diphosphate kinase activity |
| Molecular Function | GO:0016776 | phosphotransferase activity, phosphate group as acceptor |
| Molecular Function | GO:0004673 | protein histidine kinase activity |
| Molecular Function | GO:0106310 | protein serine kinase activity |
| Molecular Function | GO:0004674 | protein serine/threonine kinase activity |
| Molecular Function | GO:0043024 | ribosomal small subunit binding |
| Molecular Function | GO:0003723 | RNA binding |
| Molecular Function | GO:0120226 | succinyl-CoA binding |
| Biological Process | GO:0046356 | acetyl-CoA catabolic process |
| Biological Process | GO:0006309 | apoptotic DNA fragmentation |
| Biological Process | GO:0030154 | cell differentiation |
| Biological Process | GO:0006241 | CTP biosynthetic process |
| Biological Process | GO:0006308 | DNA catabolic process |
| Biological Process | GO:0006897 | endocytosis |
| Biological Process | GO:0006183 | GTP biosynthetic process |
| Biological Process | GO:0006720 | isoprenoid metabolic process |
| Biological Process | GO:0046042 | ITP biosynthetic process |
| Biological Process | GO:0008285 | negative regulation of cell population proliferation |
| Biological Process | GO:0009132 | nucleoside diphosphate metabolic process |
| Biological Process | GO:0009142 | nucleoside triphosphate biosynthetic process |
| Biological Process | GO:0050679 | positive regulation of epithelial cell proliferation |
| Biological Process | GO:0034214 | protein hexamerization |
| Biological Process | GO:0042981 | regulation of apoptotic process |
| Biological Process | GO:0042304 | regulation of fatty acid biosynthetic process |
| Biological Process | GO:0006228 | UTP biosynthetic process |
Reference
[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.
[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.