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Overview

Uniprot IDP16617
Protein NamePhosphoglycerate kinase 1
Gene NamePgk1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
11 SNKLTLDKLDVKGKR
131 FHVEEEGKGKDASGN
146 KVKAEPAKIDAFRAS
156 AFRASLSKLGDVYVN
184 VGVNLPQKAGGFLMK
192 AGGFLMKKELNYFAK
216 LAILGGAKVADKIQL
220 GGAKVADKIQLINNM
267 EEGAKIVKDLMAKAE
30 VDFNVPMKNNQITNN
323 CGTESSKKYAEAVAR
353 EAFARGTKSLMDEVV
361 SLMDEVVKATSRGCI
41 ITNNQRIKAAVPSIK
6 **MSLSNKLTLDKLD
91 ELKSLLGKDVLFLKD

Function

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines. In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). Acts as a protein kinase when localized to the mitochondrion where it phosphorylates pyruvate dehydrogenase kinase PDK1 to inhibit pyruvate dehydrogenase complex activity and suppress the formation of acetyl-coenzyme A from pyruvate, and consequently inhibit oxidative phosphorylation and promote glycolysis. May play a role in sperm motility

Protein Sequence

10 MSLSNKLTLD 20 KLDVKGKRVV 30 MRVDFNVPMK 40 NNQITNNQRI 50 KAAVPSIKFC 60 LDNGAKSVVL 70 MSHLGRPDGV 80 PMPDKYSLEP 90 VAAELKSLLG 100 KDVLFLKDCV 110 GSEVENACAN 120 PAAGTVILLE 130 NLRFHVEEEG 140 KGKDASGNKV 150 KAEPAKIDAF 160 RASLSKLGDV 170 YVNDAFGTAH 180 RAHSSMVGVN 190 LPQKAGGFLM 200 KKELNYFAKA 210 LESPERPFLA 220 ILGGAKVADK 230 IQLINNMLDK 240 VNEMIIGGGM 250 AFTFLKVLNN 260 MEIGTSLYDE 270 EGAKIVKDLM 280 AKAEKNGVKI 290 TLPVDFVTAD 300 KFDENAKTGQ 310 ATVASGIPAG 320 WMGLDCGTES 330 SKKYAEAVAR 340 AKQIVWNGPV 350 GVFEWEAFAR 360 GTKSLMDEVV 370 KATSRGCITI 380 IGGGDTATCC 390 AKWNTEDKVS 400 HVSTGGGASL 410 ELLEGKVLPG VDALSNV

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005615 extracellular space
Cellular Component GO:0045121 membrane raft
Cellular Component GO:0005759 mitochondrial matrix
Molecular Function GO:0043531 ADP binding
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0004618 phosphoglycerate kinase activity
Molecular Function GO:0106310 protein serine kinase activity
Molecular Function GO:0004674 protein serine/threonine kinase activity
Molecular Function GO:0047134 protein-disulfide reductase [NAD(P)H] activity
Molecular Function GO:0044325 transmembrane transporter binding
Biological Process GO:0061621 canonical glycolysis
Biological Process GO:0071456 cellular response to hypoxia
Biological Process GO:0030855 epithelial cell differentiation
Biological Process GO:0006094 gluconeogenesis
Biological Process GO:0006096 glycolytic process
Biological Process GO:0016525 negative regulation of angiogenesis
Biological Process GO:0160218 negative regulation of pyruvate decarboxylation to acetyl-CoA
Biological Process GO:0031639 plasminogen activation

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[3] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.