Search Results
Overview
| Uniprot ID | P16858 |
|---|---|
| Protein Name | Glyceraldehyde-3-phosphate dehydrogenase |
| Gene Name | Gapdh |
| Organism | Mus musculus |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 184 | HAITATQKTVDGPSG |
| 192 | TVDGPSGKLWRDGRG |
| 213 | PASTGAAKAVGKVIP |
| 217 | GAAKAVGKVIPELNG |
| 252 | CRLEKPAKYDDIKKV |
| 261 | DDIKKVVKQASEGPL |
| 70 | GKLVINGKPITIFQE |
Function
Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis (PubMed:19903941). Also shows nitrosylase activity, thereby playing a role in nuclear functions (PubMed:19903941). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (By similarity). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Biological Process | GO:0010951 | negative regulation of endopeptidase activity |
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0005829 | cytosol |
| Cellular Component | GO:0097452 | GAIT complex |
| Cellular Component | GO:0098978 | glutamatergic synapse |
| Cellular Component | GO:0031906 | late endosome lumen |
| Cellular Component | GO:0005811 | lipid droplet |
| Cellular Component | GO:0015630 | microtubule cytoskeleton |
| Cellular Component | GO:0005739 | mitochondrion |
| Cellular Component | GO:0043209 | myelin sheath |
| Cellular Component | GO:0031965 | nuclear membrane |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0005886 | plasma membrane |
| Cellular Component | GO:0099092 | postsynaptic density, intracellular component |
| Cellular Component | GO:1990904 | ribonucleoprotein complex |
| Molecular Function | GO:0019828 | aspartic-type endopeptidase inhibitor activity |
| Molecular Function | GO:0097718 | disordered domain specific binding |
| Molecular Function | GO:0019899 | enzyme binding |
| Molecular Function | GO:0004365 | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
| Molecular Function | GO:0042802 | identical protein binding |
| Molecular Function | GO:0008017 | microtubule binding |
| Molecular Function | GO:0051287 | NAD binding |
| Molecular Function | GO:0050661 | NADP binding |
| Molecular Function | GO:0035605 | peptidyl-cysteine S-nitrosylase activity |
| Biological Process | GO:0061844 | antimicrobial humoral immune response mediated by antimicrobial peptide |
| Biological Process | GO:0061621 | canonical glycolysis |
| Biological Process | GO:0071346 | cellular response to type II interferon |
| Biological Process | GO:0050832 | defense response to fungus |
| Biological Process | GO:0006094 | gluconeogenesis |
| Biological Process | GO:0046166 | glyceraldehyde-3-phosphate biosynthetic process |
| Biological Process | GO:0006096 | glycolytic process |
| Biological Process | GO:0051873 | killing by host of symbiont cells |
| Biological Process | GO:0031640 | killing of cells of another organism |
| Biological Process | GO:0000226 | microtubule cytoskeleton organization |
| Biological Process | GO:0017148 | negative regulation of translation |
| Biological Process | GO:0051402 | neuron apoptotic process |
| Biological Process | GO:0035606 | peptidyl-cysteine S-trans-nitrosylation |
| Biological Process | GO:0043123 | positive regulation of canonical NF-kappaB signal transduction |
| Biological Process | GO:0001819 | positive regulation of cytokine production |
| Biological Process | GO:0032481 | positive regulation of type I interferon production |
| Biological Process | GO:0050821 | protein stabilization |
Reference
[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.
[2] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.
[3] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.