Search Results

Overview

Uniprot IDP17174
Protein NameAspartate aminotransferase, cytoplasmic
Gene NameGOT1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
154 VFSAAGFKDIRSYRY
166 YRYWDAEKRGLDLQG
214 KQIASVMKHRFLFPF
33 REDPDPRKVNLGVGA
55 PWVLPVVKKVEQKIA
97 GDDSPALKEKRVGGV

Function

Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed:21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2-aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed:27827456)

Protein Sequence

10 MAPPSVFAEV 20 PQAQPVLVFK 30 LTADFREDPD 40 PRKVNLGVGA 50 YRTDDCHPWV 60 LPVVKKVEQK 70 IANDNSLNHE 80 YLPILGLAEF 90 RSCASRLALG 100 DDSPALKEKR 110 VGGVQSLGGT 120 GALRIGADFL 130 ARWYNGTNNK 140 NTPVYVSSPT 150 WENHNAVFSA 160 AGFKDIRSYR 170 YWDAEKRGLD 180 LQGFLNDLEN 190 APEFSIVVLH 200 ACAHNPTGID 210 PTPEQWKQIA 220 SVMKHRFLFP 230 FFDSAYQGFA 240 SGNLERDAWA 250 IRYFVSEGFE 260 FFCAQSFSKN 270 FGLYNERVGN 280 LTVVGKEPES 290 ILQVLSQMEK 300 IVRITWSNPP 310 AQGARIVAST 320 LSNPELFEEW 330 TGNVKTMADR 340 ILTMRSELRA 350 RLEALKTPGT 360 WNHITDQIGM 370 FSFTGLNPKQ 380 VEYLVNEKHI 390 YLLPSGRINV 400 SGLTTKNLDY 410 VATSIHEAVT KIQ

Gene Ontology

Classification GO ID Description
Cellular Component GO:0043679 axon terminus
Biological Process GO:0006114 glycerol biosynthetic process
Biological Process GO:0006532 L-aspartate biosynthetic process
Biological Process GO:0006533 L-aspartate catabolic process
Biological Process GO:0097054 L-glutamate biosynthetic process
Biological Process GO:0043490 malate-aspartate shuttle
Biological Process GO:0032966 negative regulation of collagen biosynthetic process
Biological Process GO:0051481 negative regulation of cytosolic calcium ion concentration
Biological Process GO:0051902 negative regulation of mitochondrial depolarization
Biological Process GO:0006107 oxaloacetate metabolic process
Biological Process GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway
Biological Process GO:0046686 response to cadmium ion
Biological Process GO:0009743 response to carbohydrate
Biological Process GO:0051384 response to glucocorticoid
Biological Process GO:0035902 response to immobilization stress
Biological Process GO:1990267 response to transition metal nanoparticle
Biological Process GO:0060290 transdifferentiation
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005634 nucleus
Molecular Function GO:0120554 2-aminobutanoate transaminase activity
Molecular Function GO:0031406 carboxylic acid binding
Molecular Function GO:0004069 L-aspartate:2-oxoglutarate aminotransferase activity
Molecular Function GO:0047801 L-cysteine transaminase activity
Molecular Function GO:0004609 phosphatidylserine decarboxylase activity
Molecular Function GO:0030170 pyridoxal phosphate binding
Biological Process GO:0006103 2-oxoglutarate metabolic process
Biological Process GO:0006531 aspartate metabolic process
Biological Process GO:0032869 cellular response to insulin stimulus
Biological Process GO:0071260 cellular response to mechanical stimulus
Biological Process GO:0006094 gluconeogenesis
Biological Process GO:0006536 glutamate metabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.