Overview
| Uniprot ID | P17225 |
|---|
| Protein Name | Polypyrimidine tract-binding protein 1 |
|---|
| Gene Name | Ptbp1 |
|---|
| Organism | Mus musculus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 434 |
SVRITLSKHQSVQLP |
Function
Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10. Binds to polypyrimidine-rich controlling element (PCE) of CFTR and promotes exon skipping of CFTR exon 9, thereby antagonizing TIA1 and its role in exon inclusion of CFTR exon 9. Plays a role in the splicing of pyruvate kinase PKM by binding repressively to a polypyrimidine tract flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform
Protein Sequence
10
MDGIVPDIAV
20
GTKRGSDELF
30
STCVSNGPFI
40
MSSSASAANG
50
NDSKKFKGDN
60
RSAGVPSRVI
70
HVRKLPSDVT
80
EGEVISLGLP
90
FGKVTNLLML
100
KGKNQAFIEM
110
NTEEAANTMV
120
NYYTSVAPVL
130
RGQPIYIQFS
140
NHKELKTDSS
150
PNQARAQAAL
160
QAVNSVQSGN
170
LALAASAAAV
180
DAGMAMAGQS
190
PVLRIIVENL
200
FYPVTLDVLH
210
QIFSKFGTVL
220
KIITFTKNNQ
230
FQALLQYADP
240
VSAQHAKLSL
250
DGQNIYNACC
260
TLRIDFSKLT
270
SLNVKYNNDK
280
SRDYTRPDLP
290
SGDSQPSLDQ
300
TMAAAFGAPG
310
IMSASPYAGA
320
GFPPTFAIPQ
330
AAGLSVPNVH
340
GALAPLAIPS
350
AAAAAAASRI
360
AIPGLAGAGN
370
SVLLVSNLNP
380
ERVTPQSLFI
390
LFGVYGDVQR
400
VKILFNKKEN
410
ALVQMADGSQ
420
AQLAMSHLNG
430
HKLHGKSVRI
440
TLSKHQSVQL
450
PREGQEDQGL
460
TKDYGSSPLH
470
RFKKPGSKNF
480
QNIFPPSATL
490
HLSNIPPSVS
500
EDDLKSLFSS
510
NGGVVKGFKF
520
FQKDRKMALI
530
QMGSVEEAVQ
540
ALIELHNHDL
550
GENHHLRVSF
SKSTI
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0044306 |
neuron projection terminus |
| Biological Process |
GO:0022008 |
neurogenesis |
| Biological Process |
GO:0070886 |
positive regulation of calcineurin-NFAT signaling cascade |
| Biological Process |
GO:0045944 |
positive regulation of transcription by RNA polymerase II |
| Biological Process |
GO:0000381 |
regulation of alternative mRNA splicing, via spliceosome |
| Biological Process |
GO:0045595 |
regulation of cell differentiation |
| Biological Process |
GO:0043484 |
regulation of RNA splicing |
| Biological Process |
GO:0008380 |
RNA splicing |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0003729 |
mRNA binding |
| Molecular Function |
GO:0036002 |
pre-mRNA binding |
| Molecular Function |
GO:0001069 |
regulatory region RNA binding |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:0043565 |
sequence-specific DNA binding |
| Molecular Function |
GO:0003697 |
single-stranded DNA binding |
| Molecular Function |
GO:0000014 |
single-stranded DNA endodeoxyribonuclease activity |
| Biological Process |
GO:0075522 |
IRES-dependent viral translational initiation |
| Biological Process |
GO:0006397 |
mRNA processing |
| Biological Process |
GO:0048025 |
negative regulation of mRNA splicing, via spliceosome |
| Biological Process |
GO:0051148 |
negative regulation of muscle cell differentiation |
| Biological Process |
GO:0045665 |
negative regulation of neuron differentiation |
| Biological Process |
GO:0033119 |
negative regulation of RNA splicing |
Reference
[1] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.