Overview
| Uniprot ID | P17710 |
| Protein Name | Hexokinase-1 |
| Gene Name | Hk1 |
| Organism | Mus musculus |
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 833 |
GQISEPLKTRGIFET |
| 884 |
TVCGVVSKRAAQLCG |
Function
Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine (By similarity). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331)
Protein Sequence
10
MGWGAPLLSR
20
MLHGPGQAGE
30
TSPVPERQSG
40
SENPASEDRR
50
PLEKQCSHHL
60
YTMGQNCQRG
70
QAVDVEPKIR
80
PPLTEEKIDK
90
YLYAMRLSDE
100
ILIDILTRFK
110
KEMKNGLSRD
120
YNPTASVKML
130
PTFVRSIPDG
140
SEKGDFIALD
150
LGGSSFRILR
160
VQVNHEKSQN
170
VSMESEVYDT
180
PENIVHGSGS
190
QLFDHVAECL
200
GDFMEKRKIK
210
DKKLPVGFTF
220
SFPCRQSKID
230
EAVLITWTKR
240
FKASGVEGAD
250
VVKLLNKAIK
260
KRGDYDANIV
270
AVVNDTVGTM
280
MTCGYDDQQC
290
EVGLIIGTGT
300
NACYMEELRH
310
IDLVEGDEGR
320
MCINTEWGAF
330
GDDGSLEDIR
340
TEFDRELDRG
350
SLNPGKQLFE
360
KMVSGMYMGE
370
LVRLILVKMA
380
KESLLFEGRI
390
TPELLTRGKF
400
TTSDVAAIET
410
DKEGVQNAKE
420
ILTRLGVEPS
430
HDDCVSVQHV
440
CTIVSFRSAN
450
LVAATLGAIL
460
NRLRDNKGTP
470
RLRTTVGVDG
480
SLYKMHPQYS
490
RRFHKTLRRL
500
VPDSDVRFLL
510
SESGSGKGAA
520
MVTAVAYRLA
530
EQHRQIEETL
540
SHFRLSKQAL
550
MEVKKKLRSE
560
MEMGLRKETN
570
SRATVKMLPS
580
YVRSIPDGTE
590
HGDFLALDLG
600
GTNFRVLLVK
610
IRSGKKRTVE
620
MHNKIYSIPL
630
EIMQGTGDEL
640
FDHIVSCISD
650
FLDYMGIKGP
660
RMPLGFTFSF
670
PCKQTSLDCG
680
ILITWTKGFK
690
ATDCVGHDVA
700
TLLRDAVKRR
710
EEFDLDVVAV
720
VNDTVGTMMT
730
CAYEEPSCEI
740
GLIVGTGSNA
750
CYMEEMKNVE
760
MVEGNQGQMC
770
INMEWGAFGD
780
NGCLDDIRTD
790
FDKVVDEYSL
800
NSGKQRFEKM
810
ISGMYLGEIV
820
RNILIDFTKK
830
GFLFRGQISE
840
PLKTRGIFET
850
KFLSQIESDR
860
LALLQVRAIL
870
QQLGLNSTCD
880
DSILVKTVCG
890
VVSKRAAQLC
900
GAGMAAVVEK
910
IRENRGLDHL
920
NVTVGVDGTL
930
YKLHPHFSRI
940
MHQTVKELSP
950
KCTVSFLLSE
960
DGSGKGAALI
970
TAVGVRLRGD
PTNA
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005901 |
caveola |
| Cellular Component |
GO:0005929 |
cilium |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0045121 |
membrane raft |
| Cellular Component |
GO:0005741 |
mitochondrial outer membrane |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0032991 |
protein-containing complex |
| Cellular Component |
GO:0097228 |
sperm principal piece |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0005536 |
D-glucose binding |
| Molecular Function |
GO:0008865 |
fructokinase activity |
| Molecular Function |
GO:0004340 |
glucokinase activity |
| Molecular Function |
GO:0047931 |
glucosamine kinase activity |
| Molecular Function |
GO:0004396 |
hexokinase activity |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0019158 |
mannokinase activity |
| Molecular Function |
GO:0042834 |
peptidoglycan binding |
| Molecular Function |
GO:0004672 |
protein kinase activity |
| Molecular Function |
GO:0044877 |
protein-containing complex binding |
| Biological Process |
GO:0061621 |
canonical glycolysis |
| Biological Process |
GO:0046835 |
carbohydrate phosphorylation |
| Biological Process |
GO:0072655 |
establishment of protein localization to mitochondrion |
| Biological Process |
GO:0006002 |
fructose 6-phosphate metabolic process |
| Biological Process |
GO:0051156 |
glucose 6-phosphate metabolic process |
| Biological Process |
GO:0006006 |
glucose metabolic process |
| Biological Process |
GO:0006096 |
glycolytic process |
| Biological Process |
GO:0006954 |
inflammatory response |
| Biological Process |
GO:0045087 |
innate immune response |
| Biological Process |
GO:0001678 |
intracellular glucose homeostasis |
| Biological Process |
GO:0072656 |
maintenance of protein location in mitochondrion |
| Biological Process |
GO:0006013 |
mannose metabolic process |
| Biological Process |
GO:0043066 |
negative regulation of apoptotic process |
| Biological Process |
GO:0018105 |
peptidyl-serine phosphorylation |
| Biological Process |
GO:0018107 |
peptidyl-threonine phosphorylation |
| Biological Process |
GO:0018108 |
peptidyl-tyrosine phosphorylation |
| Biological Process |
GO:0002720 |
positive regulation of cytokine production involved in immune response |
| Biological Process |
GO:0032731 |
positive regulation of interleukin-1 beta production |
| Biological Process |
GO:0046777 |
protein autophosphorylation |
| Biological Process |
GO:0006468 |
protein phosphorylation |
| Biological Process |
GO:0002931 |
response to ischemia |
Reference
[1] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.