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Overview

Uniprot IDP17742
Protein NamePeptidyl-prolyl cis-trans isomerase A
Gene NamePpia
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
28 SFELFADKVPKTAEN
44 RALSTGEKGFGYKGS

Function

Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (PubMed:24429998). Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation (By similarity). Activates endothelial cells (ECs) in a pro-inflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1 (By similarity). Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis (By similarity). In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2 (By similarity). Negatively regulates MAP3K5/ASK1 kinase activity, autophosphorylation and oxidative stress-induced apoptosis mediated by MAP3K5/ASK1 (By similarity). Necessary for the assembly of TARDBP in heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and regulates TARDBP binding to RNA UG repeats and TARDBP-dependent expression of HDAC6, ATG7 and VCP which are involved in clearance of protein aggregates (PubMed:25678563). Plays an important role in platelet activation and aggregation (PubMed:24429998). Regulates calcium mobilization and integrin ITGA2B:ITGB3 bidirectional signaling via increased ROS production as well as by facilitating the interaction between integrin and the cell cytoskeleton (PubMed:24429998). Binds heparan sulfate glycosaminoglycans (By similarity)

Protein Sequence

10 MVNPTVFFDI 20 TADDEPLGRV 30 SFELFADKVP 40 KTAENFRALS 50 TGEKGFGYKG 60 SSFHRIIPGF 70 MCQGGDFTRH 80 NGTGGRSIYG 90 EKFEDENFIL 100 KHTGPGILSM 110 ANAGPNTNGS 120 QFFICTAKTE 130 WLDGKHVVFG 140 KVKEGMNIVE 150 AMERFGSRNG 160 KTSKKITISD CGQL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005576 extracellular region
Cellular Component GO:0005615 extracellular space
Cellular Component GO:0043209 myelin sheath
Cellular Component GO:0005634 nucleus
Cellular Component GO:0032991 protein-containing complex
Molecular Function GO:0016018 cyclosporin A binding
Molecular Function GO:1904399 heparan sulfate binding
Molecular Function GO:0005178 integrin binding
Molecular Function GO:0003755 peptidyl-prolyl cis-trans isomerase activity
Biological Process GO:0032148 activation of protein kinase B activity
Biological Process GO:0006915 apoptotic process
Biological Process GO:0060352 cell adhesion molecule production
Biological Process GO:0034599 cellular response to oxidative stress
Biological Process GO:0034378 chylomicron assembly
Biological Process GO:0042118 endothelial cell activation
Biological Process GO:0030595 leukocyte chemotaxis
Biological Process GO:0034389 lipid droplet organization
Biological Process GO:1902176 negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
Biological Process GO:0061944 negative regulation of protein K48-linked ubiquitination
Biological Process GO:0006469 negative regulation of protein kinase activity
Biological Process GO:0001933 negative regulation of protein phosphorylation
Biological Process GO:0032873 negative regulation of stress-activated MAPK cascade
Biological Process GO:1903901 negative regulation of viral life cycle
Biological Process GO:0030182 neuron differentiation
Biological Process GO:0030593 neutrophil chemotaxis
Biological Process GO:0030168 platelet activation
Biological Process GO:0070527 platelet aggregation
Biological Process GO:0043410 positive regulation of MAPK cascade
Biological Process GO:0001934 positive regulation of protein phosphorylation
Biological Process GO:0050714 positive regulation of protein secretion
Biological Process GO:0045070 positive regulation of viral genome replication
Biological Process GO:0006457 protein folding
Biological Process GO:0000413 protein peptidyl-prolyl isomerization
Biological Process GO:2001233 regulation of apoptotic signaling pathway
Biological Process GO:0045069 regulation of viral genome replication

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.