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Overview

Uniprot IDP17764
Protein NameAcetyl-CoA acetyltransferase, mitochondrial
Gene NameAcat1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
171 ATPYGGVKLEDLIVK
199 NCAENTAKKLSISRE
210 ISREEQDKYAIGSYT
220 IGSYTRSKEAWDAGK
242 ITISVKGKPDVVVKE
248 GKPDVVVKEDEEYKR
260 YKRVDFSKVPKLKTV
265 FSKVPKLKTVFQKEN
302 AEAAQRLKVKPLARI
335 YAVPKVLKYAGLKKE

Function

This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism

Protein Sequence

10 MAALAVLHGV 20 VRRPLLRGLL 30 QEVRCLGRSY 40 ASKPTLNDVV 50 IVSATRTPIG 60 SFLGSLASQP 70 ATKLGTIAIQ 80 GAIEKAGIPK 90 EEVKEVYMGN 100 VIQGGEGQAP 110 TRQATLGAGL 120 PIATPCTTVN 130 KVCASGMKAI 140 MMASQSLMCG 150 HQDVMVAGGM 160 ESMSNVPYVM 170 SRGATPYGGV 180 KLEDLIVKDG 190 LTDVYNKIHM 200 GNCAENTAKK 210 LSISREEQDK 220 YAIGSYTRSK 230 EAWDAGKFAN 240 EITPITISVK 250 GKPDVVVKED 260 EEYKRVDFSK 270 VPKLKTVFQK 280 ENGTVTAANA 290 STLNDGAAAV 300 VLMTAEAAQR 310 LKVKPLARIA 320 AFADAAVDPI 330 DFPLAPAYAV 340 PKVLKYAGLK 350 KEDIAMWEVN 360 EAFSVVVLAN 370 IKMLEIDPQK 380 VNVHGGAVSL 390 GHPIGMSGAR 400 IVVHLAHALK 410 QGEFGLASIC 420 NGGGGASAVL IEKL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0003985 acetyl-CoA C-acetyltransferase activity
Molecular Function GO:0016453 C-acetyltransferase activity
Molecular Function GO:0034736 cholesterol O-acyltransferase activity
Molecular Function GO:0120225 coenzyme A binding
Molecular Function GO:0019899 enzyme binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0030955 potassium ion binding
Biological Process GO:0006085 acetyl-CoA biosynthetic process
Biological Process GO:0046356 acetyl-CoA catabolic process
Biological Process GO:0060612 adipose tissue development
Biological Process GO:0015937 coenzyme A biosynthetic process
Biological Process GO:0015936 coenzyme A metabolic process
Biological Process GO:0006635 fatty acid beta-oxidation
Biological Process GO:0046952 ketone body catabolic process
Biological Process GO:0006550 L-isoleucine catabolic process
Biological Process GO:0001889 liver development
Biological Process GO:0072229 metanephric proximal convoluted tubule development
Biological Process GO:1902860 propionyl-CoA biosynthetic process
Biological Process GO:0009725 response to hormone
Biological Process GO:0042594 response to starvation

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.