Search Results

Overview

Uniprot IDP17858
Protein NameATP-dependent 6-phosphofructokinase, liver type
Gene NamePFKL
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
392 YKLLAHQKPPKEKSN
677 FDRNYGTKLGVKAML
713 SACVIGLKKKAVAFS
715 CVIGLKKKAVAFSPV
726 FSPVTELKKDTDFEH

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (PubMed:22923583). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (By similarity)

Protein Sequence

10 MAAVDLEKLR 20 ASGAGKAIGV 30 LTSGGDAQGM 40 NAAVRAVTRM 50 GIYVGAKVFL 60 IYEGYEGLVE 70 GGENIKQANW 80 LSVSNIIQLG 90 GTIIGSARCK 100 AFTTREGRRA 110 AAYNLVQHGI 120 TNLCVIGGDG 130 SLTGANIFRS 140 EWGSLLEELV 150 AEGKISETTA 160 RTYSHLNIAG 170 LVGSIDNDFC 180 GTDMTIGTDS 190 ALHRIMEVID 200 AITTTAQSHQ 210 RTFVLEVMGR 220 HCGYLALVSA 230 LASGADWLFI 240 PEAPPEDGWE 250 NFMCERLGET 260 RSRGSRLNII 270 IIAEGAIDRN 280 GKPISSSYVK 290 DLVVQRLGFD 300 TRVTVLGHVQ 310 RGGTPSAFDR 320 ILSSKMGMEA 330 VMALLEATPD 340 TPACVVTLSG 350 NQSVRLPLME 360 CVQMTKEVQK 370 AMDDKRFDEA 380 TQLRGGSFEN 390 NWNIYKLLAH 400 QKPPKEKSNF 410 SLAILNVGAP 420 AAGMNAAVRS 430 AVRTGISHGH 440 TVYVVHDGFE 450 GLAKGQVQEV 460 GWHDVAGWLG 470 RGGSMLGTKR 480 TLPKGQLESI 490 VENIRIYGIH 500 ALLVVGGFEA 510 YEGVLQLVEA 520 RGRYEELCIV 530 MCVIPATISN 540 NVPGTDFSLG 550 SDTAVNAAME 560 SCDRIKQSAS 570 GTKRRVFIVE 580 TMGGYCGYLA 590 TVTGIAVGAD 600 AAYVFEDPFN 610 IHDLKVNVEH 620 MTEKMKTDIQ 630 RGLVLRNEKC 640 HDYYTTEFLY 650 NLYSSEGKGV 660 FDCRTNVLGH 670 LQQGGAPTPF 680 DRNYGTKLGV 690 KAMLWLSEKL 700 REVYRKGRVF 710 ANAPDSACVI 720 GLKKKAVAFS 730 PVTELKKDTD 740 FEHRMPREQW 750 WLSLRLMLKM 760 LAQYRISMAA 770 YVSGELEHVT 780 RRTLSMDKGF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0034774 secretory granule lumen
Cellular Component GO:0005945 6-phosphofructokinase complex
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005576 extracellular region
Cellular Component GO:1904813 ficolin-1-rich granule lumen
Cellular Component GO:0016020 membrane
Molecular Function GO:0003872 6-phosphofructokinase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0070061 fructose binding
Molecular Function GO:0070095 fructose-6-phosphate binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0019900 kinase binding
Molecular Function GO:0046872 metal ion binding
Biological Process GO:0061621 canonical glycolysis
Biological Process GO:0030388 fructose 1,6-bisphosphate metabolic process
Biological Process GO:0006002 fructose 6-phosphate metabolic process
Biological Process GO:0006096 glycolytic process
Biological Process GO:0009749 response to glucose

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[5] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[6] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[7] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[8] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.