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Overview

Uniprot IDP19367
Protein NameHexokinase-1
Gene NameHK1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
176 ITWTKRFKASGVEGA
187 VEGADVVKLLNKAIK
315 LILVKMAKEGLLFEG
333 PELLTRGKFNTSDVS
344 SDVSAIEKNKEGLHN
418 GVDGSLYKTHPQYSR
738 EYSLNAGKQRYEKMI
763 NILIDFTKKGFLFRG
77 SIPDGSEKGDFIALD
777 GQISETLKTRGIFET
885 TVKELSPKCNVSFLL

Function

Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively) (PubMed:1637300, PubMed:25316723, PubMed:27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed:27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed:27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331)

Protein Sequence

10 MIAAQLLAYY 20 FTELKDDQVK 30 KIDKYLYAMR 40 LSDETLIDIM 50 TRFRKEMKNG 60 LSRDFNPTAT 70 VKMLPTFVRS 80 IPDGSEKGDF 90 IALDLGGSSF 100 RILRVQVNHE 110 KNQNVHMESE 120 VYDTPENIVH 130 GSGSQLFDHV 140 AECLGDFMEK 150 RKIKDKKLPV 160 GFTFSFPCQQ 170 SKIDEAILIT 180 WTKRFKASGV 190 EGADVVKLLN 200 KAIKKRGDYD 210 ANIVAVVNDT 220 VGTMMTCGYD 230 DQHCEVGLII 240 GTGTNACYME 250 ELRHIDLVEG 260 DEGRMCINTE 270 WGAFGDDGSL 280 EDIRTEFDRE 290 IDRGSLNPGK 300 QLFEKMVSGM 310 YLGELVRLIL 320 VKMAKEGLLF 330 EGRITPELLT 340 RGKFNTSDVS 350 AIEKNKEGLH 360 NAKEILTRLG 370 VEPSDDDCVS 380 VQHVCTIVSF 390 RSANLVAATL 400 GAILNRLRDN 410 KGTPRLRTTV 420 GVDGSLYKTH 430 PQYSRRFHKT 440 LRRLVPDSDV 450 RFLLSESGSG 460 KGAAMVTAVA 470 YRLAEQHRQI 480 EETLAHFHLT 490 KDMLLEVKKR 500 MRAEMELGLR 510 KQTHNNAVVK 520 MLPSFVRRTP 530 DGTENGDFLA 540 LDLGGTNFRV 550 LLVKIRSGKK 560 RTVEMHNKIY 570 AIPIEIMQGT 580 GEELFDHIVS 590 CISDFLDYMG 600 IKGPRMPLGF 610 TFSFPCQQTS 620 LDAGILITWT 630 KGFKATDCVG 640 HDVVTLLRDA 650 IKRREEFDLD 660 VVAVVNDTVG 670 TMMTCAYEEP 680 TCEVGLIVGT 690 GSNACYMEEM 700 KNVEMVEGDQ 710 GQMCINMEWG 720 AFGDNGCLDD 730 IRTHYDRLVD 740 EYSLNAGKQR 750 YEKMISGMYL 760 GEIVRNILID 770 FTKKGFLFRG 780 QISETLKTRG 790 IFETKFLSQI 800 ESDRLALLQV 810 RAILQQLGLN 820 STCDDSILVK 830 TVCGVVSRRA 840 AQLCGAGMAA 850 VVDKIRENRG 860 LDRLNVTVGV 870 DGTLYKLHPH 880 FSRIMHQTVK 890 ELSPKCNVSF 900 LLSEDGSGKG 910 AALITAVGVR LRTEASS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0045121 membrane raft
Cellular Component GO:0005741 mitochondrial outer membrane
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0005536 D-glucose binding
Molecular Function GO:0008865 fructokinase activity
Molecular Function GO:0004340 glucokinase activity
Molecular Function GO:0047931 glucosamine kinase activity
Molecular Function GO:0004396 hexokinase activity
Molecular Function GO:0019158 mannokinase activity
Molecular Function GO:0042834 peptidoglycan binding
Biological Process GO:0061621 canonical glycolysis
Biological Process GO:0046835 carbohydrate phosphorylation
Biological Process GO:0072655 establishment of protein localization to mitochondrion
Biological Process GO:0006002 fructose 6-phosphate metabolic process
Biological Process GO:0009298 GDP-mannose biosynthetic process
Biological Process GO:0051156 glucose 6-phosphate metabolic process
Biological Process GO:0006006 glucose metabolic process
Biological Process GO:0006096 glycolytic process
Biological Process GO:0006954 inflammatory response
Biological Process GO:0045087 innate immune response
Biological Process GO:0001678 intracellular glucose homeostasis
Biological Process GO:0072656 maintenance of protein location in mitochondrion
Biological Process GO:0006013 mannose metabolic process
Biological Process GO:0002720 positive regulation of cytokine production involved in immune response
Biological Process GO:0032731 positive regulation of interleukin-1 beta production

Reference

[1] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.