Search Results
Overview
| Uniprot ID | P19804 |
|---|---|
| Protein Name | Nucleoside diphosphate kinase B |
| Gene Name | Nme2 |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 100 | ETNPADSKPGTIRGD |
| 12 | ERTFIAIKPDGVQRG |
| 26 | GLVGEIIKRFEQKGF |
| 31 | IIKRFEQKGFRLVAM |
| 39 | GFRLVAMKFLRASEE |
| 49 | RASEEHLKQHYIDLK |
Function
Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefore contributing to the nucleoside triphosphate homeostasis (PubMed:2832402). Also functions as a histidine protein kinase by transferring the phosphoryl group from the phosphohistidine intermediate to a histidine residue in target proteins (By similarity). Phosphorylates the GNB1 subunit of heterotrimeric G proteins at 'His-266', generating a high-energy phosphate group that promotes GTP formation and enables receptor-independent activation of heterotrimeric G proteins (By similarity). Also phosphorylates KCNN4 at 'His-358', leading to activation of its intermediate conductance calcium-activated potassium channel activity, Ca(2+) influx, and subsequent activation of B and T cells. Additionally involved in transcriptional regulation through direct DNA binding and chromatin remodeling. In this context, functions as a single-stranded DNA binding protein that binds and stabilizes the G-quadruplex (G4) structures within the nuclease hypersensitive element (NHE) III(1) region of the MYC gene promoter, facilitating recruitment of additional single-strand DNA binding proteins and activation of MYC transcription. G4 DNA-binding activity is independent of its nucleoside diphosphate kinase function and recognizes both folded and unfolded G4 structures (By similarity). With NME1, may regulate acetyl-CoA (AcCoA) usage between histone acetylation and fatty acid synthesis by targeting AcCoA release at ATP-rich, HAT-associated chromatin regions (By similarity). Also negatively regulates Rho activity by interacting with AKAP13/LBC (By similarity)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0071944 | cell periphery |
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0005829 | cytosol |
| Cellular Component | GO:0005882 | intermediate filament |
| Cellular Component | GO:0030027 | lamellipodium |
| Cellular Component | GO:0031966 | mitochondrial membrane |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0048471 | perinuclear region of cytoplasm |
| Cellular Component | GO:0001726 | ruffle |
| Molecular Function | GO:0005524 | ATP binding |
| Molecular Function | GO:0120225 | coenzyme A binding |
| Molecular Function | GO:0003677 | DNA binding |
| Molecular Function | GO:0001216 | DNA-binding transcription activator activity |
| Molecular Function | GO:0019899 | enzyme binding |
| Molecular Function | GO:0005504 | fatty acid binding |
| Molecular Function | GO:0051880 | G-quadruplex DNA binding |
| Molecular Function | GO:0019003 | GDP binding |
| Molecular Function | GO:1901363 | heterocyclic compound binding |
| Molecular Function | GO:0042802 | identical protein binding |
| Molecular Function | GO:0019215 | intermediate filament binding |
| Molecular Function | GO:0016301 | kinase activity |
| Molecular Function | GO:0046872 | metal ion binding |
| Molecular Function | GO:0004550 | nucleoside diphosphate kinase activity |
| Molecular Function | GO:0004673 | protein histidine kinase activity |
| Molecular Function | GO:0004674 | protein serine/threonine kinase activity |
| Biological Process | GO:0007189 | adenylate cyclase-activating G protein-coupled receptor signaling pathway |
| Biological Process | GO:0071398 | cellular response to fatty acid |
| Biological Process | GO:0071333 | cellular response to glucose stimulus |
| Biological Process | GO:0034599 | cellular response to oxidative stress |
| Biological Process | GO:0006241 | CTP biosynthetic process |
| Biological Process | GO:0006351 | DNA-templated transcription |
| Biological Process | GO:0006183 | GTP biosynthetic process |
| Biological Process | GO:0007229 | integrin-mediated signaling pathway |
| Biological Process | GO:0046042 | ITP biosynthetic process |
| Biological Process | GO:0043066 | negative regulation of apoptotic process |
| Biological Process | GO:0002762 | negative regulation of myeloid leukocyte differentiation |
| Biological Process | GO:0009142 | nucleoside triphosphate biosynthetic process |
| Biological Process | GO:0045893 | positive regulation of DNA-templated transcription |
| Biological Process | GO:2000144 | positive regulation of DNA-templated transcription initiation |
| Biological Process | GO:0050679 | positive regulation of epithelial cell proliferation |
| Biological Process | GO:0045618 | positive regulation of keratinocyte differentiation |
| Biological Process | GO:0010976 | positive regulation of neuron projection development |
| Biological Process | GO:0045944 | positive regulation of transcription by RNA polymerase II |
| Biological Process | GO:0046777 | protein autophosphorylation |
| Biological Process | GO:0042981 | regulation of apoptotic process |
| Biological Process | GO:0045682 | regulation of epidermis development |
| Biological Process | GO:0042304 | regulation of fatty acid biosynthetic process |
| Biological Process | GO:0060416 | response to growth hormone |
| Biological Process | GO:0006228 | UTP biosynthetic process |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.
[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.