Overview
| Uniprot ID | P20336 |
|---|
| Protein Name | Ras-related protein Rab-3A |
|---|
| Gene Name | RAB3A |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 173 |
AKDNINVKQTFERLV |
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed:2501306). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:2501306). RAB3A plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane (PubMed:2501306). Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane (By similarity). Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed (By similarity). Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells (By similarity). Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9 (PubMed:27325790). Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1 (PubMed:22248876, PubMed:30599141). Also plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT (By similarity)
Protein Sequence
10
MASATDSRYG
20
QKESSDQNFD
30
YMFKILIIGN
40
SSVGKTSFLF
50
RYADDSFTPA
60
FVSTVGIDFK
70
VKTIYRNDKR
80
IKLQIWDTAG
90
QERYRTITTA
100
YYRGAMGFIL
110
MYDITNEESF
120
NAVQDWSTQI
130
KTYSWDNAQV
140
LLVGNKCDME
150
DERVVSSERG
160
RQLADHLGFE
170
FFEASAKDNI
180
NVKQTFERLV
190
DVICEKMSES
200
LDTADPAVTG
210
AKQGPQLSDQ
220
QVPPHQDCAC
Gene Ontology
| Classification |
GO ID |
Description |
| Biological Process |
GO:0099161 |
regulation of presynaptic dense core granule exocytosis |
| Cellular Component |
GO:0001669 |
acrosomal vesicle |
| Cellular Component |
GO:0030424 |
axon |
| Cellular Component |
GO:0060201 |
clathrin-sculpted acetylcholine transport vesicle membrane |
| Cellular Component |
GO:0061202 |
clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane |
| Cellular Component |
GO:0060203 |
clathrin-sculpted glutamate transport vesicle membrane |
| Cellular Component |
GO:0070083 |
clathrin-sculpted monoamine transport vesicle membrane |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005768 |
endosome |
| Cellular Component |
GO:1903561 |
extracellular vesicle |
| Cellular Component |
GO:0005764 |
lysosome |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0098794 |
postsynapse |
| Cellular Component |
GO:0098793 |
presynapse |
| Cellular Component |
GO:0048786 |
presynaptic active zone |
| Cellular Component |
GO:0030667 |
secretory granule membrane |
| Cellular Component |
GO:0008021 |
synaptic vesicle |
| Cellular Component |
GO:0030672 |
synaptic vesicle membrane |
| Cellular Component |
GO:0043195 |
terminal bouton |
| Molecular Function |
GO:0003925 |
G protein activity |
| Molecular Function |
GO:0005525 |
GTP binding |
| Molecular Function |
GO:0030742 |
GTP-dependent protein binding |
| Molecular Function |
GO:0003924 |
GTPase activity |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0031489 |
myosin V binding |
| Molecular Function |
GO:0030674 |
protein-macromolecule adaptor activity |
| Biological Process |
GO:0060478 |
acrosomal vesicle exocytosis |
| Biological Process |
GO:0007409 |
axonogenesis |
| Biological Process |
GO:0045054 |
constitutive secretory pathway |
| Biological Process |
GO:0061670 |
evoked neurotransmitter secretion |
| Biological Process |
GO:0006887 |
exocytosis |
| Biological Process |
GO:0030073 |
insulin secretion |
| Biological Process |
GO:0032418 |
lysosome localization |
| Biological Process |
GO:0001778 |
plasma membrane repair |
| Biological Process |
GO:0045921 |
positive regulation of exocytosis |
| Biological Process |
GO:1903307 |
positive regulation of regulated secretory pathway |
| Biological Process |
GO:0045055 |
regulated exocytosis |
| Biological Process |
GO:1905684 |
regulation of plasma membrane repair |
| Biological Process |
GO:0048172 |
regulation of short-term neuronal synaptic plasticity |
| Biological Process |
GO:0031630 |
regulation of synaptic vesicle fusion to presynaptic active zone membrane |
| Biological Process |
GO:0097091 |
synaptic vesicle clustering |
| Biological Process |
GO:0016079 |
synaptic vesicle exocytosis |
| Biological Process |
GO:0036465 |
synaptic vesicle recycling |
| Biological Process |
GO:0048489 |
synaptic vesicle transport |
Reference
[1] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.