Search Results

Overview

Uniprot ID	P20674
Protein Name	Cytochrome c oxidase subunit 5A, mitochondrial
Gene Name	COX5A
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
113	VRILEVVKDKAGPHK
72	IDAWELRKGINTLVT

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix

Protein Sequence

10 MLGAALRRCA 20 VAATTRADPR 30 GLLHSARTPG 40 PAVAIQSVRC 50 YSHGSQETDE 60 EFDARWVTYF 70 NKPDIDAWEL 80 RKGINTLVTY 90 DMVPEPKIID 100 AALRACRRLN 110 DFASTVRILE 120 VVKDKAGPHK 130 EIYPYVIQEL 140 RPTLNELGIS 150 TPEELGLDKV

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005743	mitochondrial inner membrane
Cellular Component	GO:0005758	mitochondrial intermembrane space
Cellular Component	GO:0031966	mitochondrial membrane
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0045277	respiratory chain complex IV
Molecular Function	GO:0004129	cytochrome-c oxidase activity
Molecular Function	GO:0009055	electron transfer activity
Molecular Function	GO:0046872	metal ion binding
Biological Process	GO:0045333	cellular respiration
Biological Process	GO:0006123	mitochondrial electron transport, cytochrome c to oxygen

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[3] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.