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Overview

Uniprot IDP20788
Protein NameCytochrome b-c1 complex subunit Rieske, mitochondrial
Gene NameUqcrfs1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
101 AEVLDSTKSSKESSE
163 LSDIPEGKNMAFKWR
182 FVRHRTKKEIDQEAA

Function

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. The Rieske protein is a catalytic core subunit containing a [2Fe-2S] iron-sulfur cluster. It cycles between 2 conformational states during catalysis to transfer electrons from the quinol bound in the Q(0) site in cytochrome b to cytochrome c1 (By similarity). Incorporation of UQCRFS1 is the penultimate step in complex III assembly (By similarity)

Protein Sequence

10 MLSVAARSGP 20 FAPVLSATSR 30 GVAGALRPLL 40 QSAVPATSEP 50 PVLDVKRPFL 60 CRESLSGQAA 70 TRPLVATVGL 80 NVPASVRYSH 90 TDIKVPDFSD 100 YRRAEVLDST 110 KSSKESSEAR 120 KGFSYLVTAT 130 TTVGVAYAAK 140 NAVSQFVSSM 150 SASADVLAMS 160 KIEIKLSDIP 170 EGKNMAFKWR 180 GKPLFVRHRT 190 KKEIDQEAAV 200 EVSQLRDPQH 210 DLERVKKPEW 220 VILIGVCTHL 230 GCVPIANAGD 240 FGGYYCPCHG 250 SHYDASGRIR 260 KGPAPLNLEV 270 PTYEFTSGDV VVVG

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0031966 mitochondrial membrane
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0045275 respiratory chain complex III
Molecular Function GO:0051537 2 iron, 2 sulfur cluster binding
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0016491 oxidoreductase activity
Molecular Function GO:0044877 protein-containing complex binding
Molecular Function GO:0008121 quinol-cytochrome-c reductase activity
Biological Process GO:0006122 mitochondrial electron transport, ubiquinol to cytochrome c
Biological Process GO:0034551 mitochondrial respiratory chain complex III assembly
Biological Process GO:0022904 respiratory electron transport chain
Biological Process GO:0009725 response to hormone
Biological Process GO:0009410 response to xenobiotic stimulus

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.