Search Results

Overview

Uniprot IDP20810
Protein NameCalpastatin
Gene NameCAST
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
132 GDKKKEKKSLTPAVP
143 PAVPVESKPDKPSGK
204 REVTIPPKYRELLAK
212 YRELLAKKEGITGPP
250 SPTAAGKKTEKEEST
253 AAGKKTEKEESTEVL
261 EESTEVLKAQSAGTV
283 EKKRKVEKDTMSDQA
319 IKEVDEAKAKEEKLE
396 TEKTEESKAAAPAPV
43 EKKSQSTKLSVVHEK
50 KLSVVHEKKSQEGKP
51 LSVVHEKKSQEGKPK
58 KSQEGKPKEHTEPKS
64 PKEHTEPKSLPKQAS
68 TEPKSLPKQASDTGS
684 KKAASSSKAPKNGGK
687 ASSSKAPKNGGKAKD
691 KAPKNGGKAKDSAKT

Function

Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue

Protein Sequence

10 MNPTETKAIP 20 VSQQMEGPHL 30 PNKKKHKKQA 40 VKTEPEKKSQ 50 STKLSVVHEK 60 KSQEGKPKEH 70 TEPKSLPKQA 80 SDTGSNDAHN 90 KKAVSRSAEQ 100 QPSEKSTEPK 110 TKPQDMISAG 120 GESVAGITAI 130 SGKPGDKKKE 140 KKSLTPAVPV 150 ESKPDKPSGK 160 SGMDAALDDL 170 IDTLGGPEET 180 EEENTTYTGP 190 EVSDPMSSTY 200 IEELGKREVT 210 IPPKYRELLA 220 KKEGITGPPA 230 DSSKPIGPDD 240 AIDALSSDFT 250 CGSPTAAGKK 260 TEKEESTEVL 270 KAQSAGTVRS 280 AAPPQEKKRK 290 VEKDTMSDQA 300 LEALSASLGT 310 RQAEPELDLR 320 SIKEVDEAKA 330 KEEKLEKCGE 340 DDETIPSEYR 350 LKPATDKDGK 360 PLLPEPEEKP 370 KPRSESELID 380 ELSEDFDRSE 390 CKEKPSKPTE 400 KTEESKAAAP 410 APVSEAVCRT 420 SMCSIQSAPP 430 EPATLKGTVP 440 DDAVEALADS 450 LGKKEADPED 460 GKPVMDKVKE 470 KAKEEDREKL 480 GEKEETIPPD 490 YRLEEVKDKD 500 GKPLLPKESK 510 EQLPPMSEDF 520 LLDALSEDFS 530 GPQNASSLKF 540 EDAKLAAAIS 550 EVVSQTPAST 560 TQAGAPPRDT 570 SQSDKDLDDA 580 LDKLSDSLGQ 590 RQPDPDENKP 600 MEDKVKEKAK 610 AEHRDKLGER 620 DDTIPPEYRH 630 LLDDNGQDKP 640 VKPPTKKSED 650 SKKPADDQDP 660 IDALSGDLDS 670 CPSTTETSQN 680 TAKDKCKKAA 690 SSSKAPKNGG 700 KAKDSAKTTE ETSKPKDD

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0016020 membrane
Cellular Component GO:0005886 plasma membrane
Molecular Function GO:0045296 cadherin binding
Molecular Function GO:0010859 calcium-dependent cysteine-type endopeptidase inhibitor activity
Molecular Function GO:0004866 endopeptidase inhibitor activity
Molecular Function GO:0003723 RNA binding
Biological Process GO:2000675 negative regulation of type B pancreatic cell apoptotic process
Biological Process GO:1990709 presynaptic active zone organization

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[5] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[6] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[7] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[8] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.