Search Results

Overview

Uniprot IDP21399
Protein NameCytoplasmic aconitate hydratase
Gene NameACO1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
141 RRADSLQKNQDLEFE
20 DPVQPGKKFFNLNKL
26 KKFFNLNKLEDSRYG
335 EKLKYIKKYLQAVGM
379 GPKRPQDKVAVSDMK
387 VAVSDMKKDFESCLG
396 FESCLGAKQGFKGFQ
56 NCDEFLVKKQDIENI
57 CDEFLVKKQDIENIL
572 TIRIDFEKEPLGVNA
639 KLFFWNSKSTYIKSP
852 PQMKVQVKLDTGKTF

Function

Bifunctional iron sensor that switches between 2 activities depending on iron availability (PubMed:1281544, PubMed:1946430, PubMed:8041788). Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization (PubMed:1281544, PubMed:1946430, PubMed:23891004, PubMed:8041788). Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA (PubMed:1281544, PubMed:1946430, PubMed:23891004, PubMed:8041788)

Protein Sequence

10 MSNPFAHLAE 20 PLDPVQPGKK 30 FFNLNKLEDS 40 RYGRLPFSIR 50 VLLEAAIRNC 60 DEFLVKKQDI 70 ENILHWNVTQ 80 HKNIEVPFKP 90 ARVILQDFTG 100 VPAVVDFAAM 110 RDAVKKLGGD 120 PEKINPVCPA 130 DLVIDHSIQV 140 DFNRRADSLQ 150 KNQDLEFERN 160 RERFEFLKWG 170 SQAFHNMRII 180 PPGSGIIHQV 190 NLEYLARVVF 200 DQDGYYYPDS 210 LVGTDSHTTM 220 IDGLGILGWG 230 VGGIEAEAVM 240 LGQPISMVLP 250 QVIGYRLMGK 260 PHPLVTSTDI 270 VLTITKHLRQ 280 VGVVGKFVEF 290 FGPGVAQLSI 300 ADRATIANMC 310 PEYGATAAFF 320 PVDEVSITYL 330 VQTGRDEEKL 340 KYIKKYLQAV 350 GMFRDFNDPS 360 QDPDFTQVVE 370 LDLKTVVPCC 380 SGPKRPQDKV 390 AVSDMKKDFE 400 SCLGAKQGFK 410 GFQVAPEHHN 420 DHKTFIYDNT 430 EFTLAHGSVV 440 IAAITSCTNT 450 SNPSVMLGAG 460 LLAKKAVDAG 470 LNVMPYIKTS 480 LSPGSGVVTY 490 YLQESGVMPY 500 LSQLGFDVVG 510 YGCMTCIGNS 520 GPLPEPVVEA 530 ITQGDLVAVG 540 VLSGNRNFEG 550 RVHPNTRANY 560 LASPPLVIAY 570 AIAGTIRIDF 580 EKEPLGVNAK 590 GQQVFLKDIW 600 PTRDEIQAVE 610 RQYVIPGMFK 620 EVYQKIETVN 630 ESWNALATPS 640 DKLFFWNSKS 650 TYIKSPPFFE 660 NLTLDLQPPK 670 SIVDAYVLLN 680 LGDSVTTDHI 690 SPAGNIARNS 700 PAARYLTNRG 710 LTPREFNSYG 720 SRRGNDAVMA 730 RGTFANIRLL 740 NRFLNKQAPQ 750 TIHLPSGEIL 760 DVFDAAERYQ 770 QAGLPLIVLA 780 GKEYGAGSSR 790 DWAAKGPFLL 800 GIKAVLAESY 810 ERIHRSNLVG 820 MGVIPLEYLP 830 GENADALGLT 840 GQERYTIIIP 850 ENLKPQMKVQ 860 VKLDTGKTFQ 870 AVMRFDTDVE 880 LTYFLNGGIL NYMIRKMAK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005794 Golgi apparatus
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0051538 3 iron, 4 sulfur cluster binding
Molecular Function GO:0051539 4 iron, 4 sulfur cluster binding
Molecular Function GO:0003994 aconitate hydratase activity
Molecular Function GO:0030350 iron-responsive element binding
Molecular Function GO:0051536 iron-sulfur cluster binding
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0000900 mRNA regulatory element binding translation repressor activity
Molecular Function GO:0003723 RNA binding
Biological Process GO:0006101 citrate metabolic process
Biological Process GO:0006879 intracellular iron ion homeostasis
Biological Process GO:0006740 NADPH regeneration
Biological Process GO:0010040 response to iron(II) ion
Biological Process GO:0006099 tricarboxylic acid cycle

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.