Overview
| Uniprot ID | P22059 |
|---|
| Protein Name | Oxysterol-binding protein 1 |
|---|
| Gene Name | OSBP |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 340 |
PGNVGSGKDQCCSGK |
Function
Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol (cholesterol) with phosphatidylinositol 4-phosphate (PI4P, 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)), delivering sterol to the Golgi in exchange for PI4P, which is subsequently degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum (PubMed:24209621, PubMed:28978670). Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol (PubMed:15746430, PubMed:17428193). Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly (PubMed:15746430). Regulates cholesterol efflux by decreasing the stability of the relatively short lived ABCA1 protein (an important point of control for cholesterol efflux activity) (PubMed:18450749)
Protein Sequence
10
MAATELRGVV
20
GPGPAAIAAL
30
GGGGAGPPVV
40
GGGGGRGDAG
50
PGSGAASGTV
60
VAAAAGGPGP
70
GAGGVAAAGP
80
APAPPTGGSG
90
GSGAGGSGSA
100
REGWLFKWTN
110
YIKGYQRRWF
120
VLSNGLLSYY
130
RSKAEMRHTC
140
RGTINLATAN
150
ITVEDSCNFI
160
ISNGGAQTYH
170
LKASSEVERQ
180
RWVTALELAK
190
AKAVKMLAES
200
DESGDEESVS
210
QTDKTELQNT
220
LRTLSSKVED
230
LSTCNDLIAK
240
HGTALQRSLS
250
ELESLKLPAE
260
SNEKIKQVNE
270
RATLFRITSN
280
AMINACRDFL
290
MLAQTHSKKW
300
QKSLQYERDQ
310
RIRLEETLEQ
320
LAKQHNHLER
330
AFRGATVLPA
340
NTPGNVGSGK
350
DQCCSGKGDM
360
SDEDDENEFF
370
DAPEIITMPE
380
NLGHKRTGSN
390
ISGASSDISL
400
DEQYKHQLEE
410
TKKEKRTRIP
420
YKPNYSLNLW
430
SIMKNCIGKE
440
LSKIPMPVNF
450
NEPLSMLQRL
460
TEDLEYHELL
470
DRAAKCENSL
480
EQLCYVAAFT
490
VSSYSTTVFR
500
TSKPFNPLLG
510
ETFELDRLEE
520
NGYRSLCEQV
530
SHHPPAAAHH
540
AESKNGWTLR
550
QEIKITSKFR
560
GKYLSIMPLG
570
TIHCIFHATG
580
HHYTWKKVTT
590
TVHNIIVGKL
600
WIDQSGEIDI
610
VNHKTGDKCN
620
LKFVPYSYFS
630
RDVARKVTGE
640
VTDPSGKVHF
650
ALLGTWDEKM
660
ECFKVQPVIG
670
ENGGDARQRG
680
HEAEESRVML
690
WKRNPLPKNA
700
ENMYYFSELA
710
LTLNAWESGT
720
APTDSRLRPD
730
QRLMENGRWD
740
EANAEKQRLE
750
EKQRLSRKKR
760
EAEAMKATED
770
GTPYDPYKAL
780
WFERKKDPVT
790
KELTHIYRGE
800
YWECKEKQDW
SSCPDIF
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0160258 |
endoplasmic reticulum-trans-Golgi network membrane contact site |
| Cellular Component |
GO:0005794 |
Golgi apparatus |
| Cellular Component |
GO:0000139 |
Golgi membrane |
| Cellular Component |
GO:0016020 |
membrane |
| Cellular Component |
GO:0097038 |
perinuclear endoplasmic reticulum |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0005802 |
trans-Golgi network |
| Molecular Function |
GO:0120013 |
lipid transfer activity |
| Molecular Function |
GO:0008142 |
oxysterol binding |
| Molecular Function |
GO:0070273 |
phosphatidylinositol-4-phosphate binding |
| Molecular Function |
GO:0160291 |
phosphatidylinositol-4-phosphate-cholesterol exchange activity |
| Molecular Function |
GO:0019904 |
protein domain specific binding |
| Molecular Function |
GO:0032934 |
sterol binding |
| Molecular Function |
GO:0120015 |
sterol transfer activity |
| Biological Process |
GO:0006699 |
bile acid biosynthetic process |
| Biological Process |
GO:0035627 |
ceramide transport |
| Biological Process |
GO:0032367 |
intracellular cholesterol transport |
| Biological Process |
GO:0006869 |
lipid transport |
| Biological Process |
GO:0015914 |
phospholipid transport |
| Biological Process |
GO:0035774 |
positive regulation of insulin secretion involved in cellular response to glucose stimulus |
| Biological Process |
GO:1904411 |
positive regulation of secretory granule organization |
| Biological Process |
GO:0006686 |
sphingomyelin biosynthetic process |
| Biological Process |
GO:0015918 |
sterol transport |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.