Search Results

Overview

Uniprot ID	P22061
Protein Name	Protein-L-isoaspartate(D-aspartate) O-methyltransferase
Gene Name	PCMT1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
125	DSVNNVRKDDPTLLS
4	****MAWKSGGASHS

Function

Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins (PubMed:3167043, PubMed:6469980). Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (By similarity)

Protein Sequence

10 MAWKSGGASH 20 SELIHNLRKN 30 GIIKTDKVFE 40 VMLATDRSHY 50 AKCNPYMDSP 60 QSIGFQATIS 70 APHMHAYALE 80 LLFDQLHEGA 90 KALDVGSGSG 100 ILTACFARMV 110 GCTGKVIGID 120 HIKELVDDSV 130 NNVRKDDPTL 140 LSSGRVQLVV 150 GDGRMGYAEE 160 APYDAIHVGA 170 AAPVVPQALI 180 DQLKPGGRLI 190 LPVGPAGGNQ 200 MLEQYDKLQD 210 GSIKMKPLMG 220 VIYVPLTDKE KQWSRWK

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0070062	extracellular exosome
Cellular Component	GO:1903561	extracellular vesicle
Molecular Function	GO:0045296	cadherin binding
Molecular Function	GO:0004719	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity
Biological Process	GO:0006479	protein methylation
Biological Process	GO:0030091	protein repair

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[4] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[5] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[6] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[7] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.