Search Results

Overview

Uniprot IDP22087
Protein NamerRNA 2'-O-methyltransferase fibrillarin
Gene NameFBL
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
102 GVFICRGKEDALVTK
121 GESVYGEKRVSISEG
143 AWNPFRSKLAAAILG
318 GVYRPPPKVKN****

Function

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed:24352239, PubMed:30540930, PubMed:32017898). Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed:30540930). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed:32017898). U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed:32017898). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239, PubMed:30540930). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797)

Protein Sequence

10 MKPGFSPRGG 20 GFGGRGGFGD 30 RGGRGGRGGF 40 GGGRGRGGGF 50 RGRGRGGGGG 60 GGGGGGGGRG 70 GGGFHSGGNR 80 GRGRGGKRGN 90 QSGKNVMVEP 100 HRHEGVFICR 110 GKEDALVTKN 120 LVPGESVYGE 130 KRVSISEGDD 140 KIEYRAWNPF 150 RSKLAAAILG 160 GVDQIHIKPG 170 AKVLYLGAAS 180 GTTVSHVSDI 190 VGPDGLVYAV 200 EFSHRSGRDL 210 INLAKKRTNI 220 IPVIEDARHP 230 HKYRMLIAMV 240 DVIFADVAQP 250 DQTRIVALNA 260 HTFLRNGGHF 270 VISIKANCID 280 STASAEAVFA 290 SEVKKMQQEN 300 MKPQEQLTLE 310 PYERDHAVVV 320 GVYRPPPKVK N

Gene Ontology

Classification GO ID Description
Cellular Component GO:0016020 membrane
Cellular Component GO:0031428 box C/D methylation guide snoRNP complex
Cellular Component GO:0015030 Cajal body
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0001650 fibrillar center
Cellular Component GO:0001652 granular component
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0032040 small-subunit processome
Molecular Function GO:0051117 ATPase binding
Molecular Function GO:1990259 histone H2AQ104 methyltransferase activity
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0008649 rRNA methyltransferase activity
Molecular Function GO:0001094 TFIID-class transcription factor complex binding
Molecular Function GO:0180021 U6 snRNA 2'-O-ribose methyltransferase activity
Biological Process GO:0000494 box C/D sno(s)RNA 3'-end processing
Biological Process GO:0030490 maturation of SSU-rRNA
Biological Process GO:0016556 mRNA modification
Biological Process GO:0001649 osteoblast differentiation
Biological Process GO:0042274 ribosomal small subunit biogenesis
Biological Process GO:0000451 rRNA 2'-O-methylation
Biological Process GO:0031167 rRNA methylation
Biological Process GO:0006364 rRNA processing
Biological Process GO:0048254 snoRNA localization

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[3] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.