Overview
| Uniprot ID | P22509 |
|---|
| Protein Name | rRNA 2'-O-methyltransferase fibrillarin |
|---|
| Gene Name | Fbl |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 108 |
GVFICRGKEDALVTK |
| 127 |
GESVYGEKRVSISEG |
| 324 |
GVYRPPPKAKN**** |
Function
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (By similarity). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes. U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (By similarity). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (By similarity)
Protein Sequence
10
MKPGFSPRGG
20
GFGGRGGFGD
30
RGGRGGGRGG
40
RGGFGGGRGG
50
FGGGGRGRGG
60
GGGGFRGRGG
70
GGGRGGGFQS
80
GGGRGRGGGR
90
GGKRGNQSGK
100
NVMVEPHRHE
110
GVFICRGKED
120
ALVTKNLVPG
130
ESVYGEKRVS
140
ISEGDDKIEY
150
RAWNPFRSKL
160
AAAILGGVDQ
170
IHIKPGAKVL
180
YLGAASGTTV
190
SHVSDIVGPD
200
GLVYAVEFSH
210
RSGRDLINLA
220
KKRTNIIPVI
230
EDARHPHKYR
240
MLIAMVDVIF
250
ADVAQPDQTR
260
IVALNAHTFL
270
RNGGHFVISI
280
KANCIDSTAS
290
AEAVFASEVK
300
KMQQENMKPQ
310
EQLTLEPYER
320
DHAVVVGVYR
PPPKAKN
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0031428 |
box C/D methylation guide snoRNP complex |
| Cellular Component |
GO:0015030 |
Cajal body |
| Cellular Component |
GO:0005694 |
chromosome |
| Cellular Component |
GO:0001651 |
dense fibrillar component |
| Cellular Component |
GO:0001650 |
fibrillar center |
| Cellular Component |
GO:0001652 |
granular component |
| Cellular Component |
GO:0005730 |
nucleolus |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0032040 |
small-subunit processome |
| Molecular Function |
GO:0051117 |
ATPase binding |
| Molecular Function |
GO:1990259 |
histone H2AQ104 methyltransferase activity |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:0008649 |
rRNA methyltransferase activity |
| Molecular Function |
GO:0001094 |
TFIID-class transcription factor complex binding |
| Molecular Function |
GO:0180021 |
U6 snRNA 2'-O-ribose methyltransferase activity |
| Biological Process |
GO:0000494 |
box C/D sno(s)RNA 3'-end processing |
| Biological Process |
GO:0042274 |
ribosomal small subunit biogenesis |
| Biological Process |
GO:0031167 |
rRNA methylation |
| Biological Process |
GO:0016074 |
sno(s)RNA metabolic process |
| Biological Process |
GO:0048254 |
snoRNA localization |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.