Search Results

Overview

Uniprot IDP23141
Protein NameLiver carboxylesterase 1
Gene NameCES1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
105 SELFTNRKENIPLKL
257 ALTSVLVKKGDVKPL
258 LTSVLVKKGDVKPLA
262 LVKKGDVKPLAEQIA
275 IAITAGCKTTTSAVM
289 MVHCLRQKTEEELLE
300 ELLETTLKMKFLSLD
302 LETTLKMKFLSLDLQ
384 TAMSLLWKSYPLVCI
393 YPLVCIAKELIPEAT
402 LIPEATEKYLGGTDD
412 GGTDDTVKKKDLFLD
460 PSFSSDMKPKTVIGD
494 EEEIRLSKMVMKFWA
538 ANTQAAQKLKDKEVA
542 AAQKLKDKEVAFWTN
553 FWTNLFAKKAVEKPP
554 WTNLFAKKAVEKPPQ
558 FAKKAVEKPPQTEHI

Function

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed:18762277, PubMed:7980644, PubMed:9169443, PubMed:9490062). Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester (PubMed:18762277, PubMed:7980644, PubMed:9169443, PubMed:9490062). Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine (PubMed:7980644). Catalyzes the transesterification of cocaine to form cocaethylene (PubMed:7980644). Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate (PubMed:7980644). Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins (PubMed:21049984). Hydrolyzes cellular cholesteryl esters to free cholesterols and promotes reverse cholesterol transport (RCT) by facilitating both the initial and final steps in the process (PubMed:11015575, PubMed:16024911, PubMed:16971496, PubMed:18762277). First of all, allows free cholesterol efflux from macrophages to extracellular cholesterol acceptors and secondly, releases free cholesterol from lipoprotein-delivered cholesteryl esters in the liver for bile acid synthesis or direct secretion into the bile (PubMed:16971496, PubMed:18599737, PubMed:18762277)

Protein Sequence

10 MWLRAFILAT 20 LSASAAWGHP 30 SSPPVVDTVH 40 GKVLGKFVSL 50 EGFAQPVAIF 60 LGIPFAKPPL 70 GPLRFTPPQP 80 AEPWSFVKNA 90 TSYPPMCTQD 100 PKAGQLLSEL 110 FTNRKENIPL 120 KLSEDCLYLN 130 IYTPADLTKK 140 NRLPVMVWIH 150 GGGLMVGAAS 160 TYDGLALAAH 170 ENVVVVTIQY 180 RLGIWGFFST 190 GDEHSRGNWG 200 HLDQVAALRW 210 VQDNIASFGG 220 NPGSVTIFGE 230 SAGGESVSVL 240 VLSPLAKNLF 250 HRAISESGVA 260 LTSVLVKKGD 270 VKPLAEQIAI 280 TAGCKTTTSA 290 VMVHCLRQKT 300 EEELLETTLK 310 MKFLSLDLQG 320 DPRESQPLLG 330 TVIDGMLLLK 340 TPEELQAERN 350 FHTVPYMVGI 360 NKQEFGWLIP 370 MQLMSYPLSE 380 GQLDQKTAMS 390 LLWKSYPLVC 400 IAKELIPEAT 410 EKYLGGTDDT 420 VKKKDLFLDL 430 IADVMFGVPS 440 VIVARNHRDA 450 GAPTYMYEFQ 460 YRPSFSSDMK 470 PKTVIGDHGD 480 ELFSVFGAPF 490 LKEGASEEEI 500 RLSKMVMKFW 510 ANFARNGNPN 520 GEGLPHWPEY 530 NQKEGYLQIG 540 ANTQAAQKLK 550 DKEVAFWTNL 560 FAKKAVEKPP QTEHIEL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0005788 endoplasmic reticulum lumen
Cellular Component GO:0005811 lipid droplet
Molecular Function GO:0106435 carboxylesterase activity
Molecular Function GO:0047374 methylumbelliferyl-acetate deacetylase activity
Molecular Function GO:0050253 retinyl-palmitate esterase activity
Molecular Function GO:0004771 sterol ester esterase activity
Biological Process GO:0071397 cellular response to cholesterol
Biological Process GO:0071404 cellular response to low-density lipoprotein particle stimulus
Biological Process GO:0006695 cholesterol biosynthetic process
Biological Process GO:0090122 cholesterol ester hydrolysis involved in cholesterol transport
Biological Process GO:0042632 cholesterol homeostasis
Biological Process GO:0008203 cholesterol metabolic process
Biological Process GO:0030855 epithelial cell differentiation
Biological Process GO:0051791 medium-chain fatty acid metabolic process
Biological Process GO:0010887 negative regulation of cholesterol storage
Biological Process GO:0010875 positive regulation of cholesterol efflux
Biological Process GO:0090205 positive regulation of cholesterol metabolic process
Biological Process GO:0070857 regulation of bile acid biosynthetic process
Biological Process GO:0120188 regulation of bile acid secretion
Biological Process GO:0009636 response to toxic substance
Biological Process GO:0043691 reverse cholesterol transport
Biological Process GO:0019626 short-chain fatty acid catabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.