Search Results

Overview

Uniprot IDP23506
Protein NameProtein-L-isoaspartate(D-aspartate) O-methyltransferase
Gene NamePcmt1
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
4 ****MAWKSGGASHS

Function

Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins (PubMed:12023972, PubMed:16923807, PubMed:20424163). Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (PubMed:16923807, PubMed:20424163)

Protein Sequence

10 MAWKSGGASH 20 SELIHNLRKN 30 GIIKTDKVFE 40 VMLATDRSHY 50 AKSNPYMDSP 60 QSIGFQATIS 70 APHMHAYALE 80 LLFDQLHEGA 90 KALDVGSGSG 100 ILTACFARMV 110 GNSGKVIGID 120 HIKELVDDSI 130 TNVKKDDPML 140 LSSGRVRLVV 150 GDGRMGYAEE 160 APYDAIHVGA 170 AAPVVPQALI 180 DQLKPGGRLI 190 LPVGPAGGNQ 200 MLEQYDKLQD 210 GSVKMKPLMG 220 VIYVPLTDKE KQWSRWK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0016323 basolateral plasma membrane
Cellular Component GO:0031526 brush border membrane
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005615 extracellular space
Cellular Component GO:0043204 perikaryon
Molecular Function GO:0004719 protein-L-isoaspartate (D-aspartate) O-methyltransferase activity
Molecular Function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
Biological Process GO:0010667 negative regulation of cardiac muscle cell apoptotic process
Biological Process GO:0006479 protein methylation
Biological Process GO:0046498 S-adenosylhomocysteine metabolic process
Biological Process GO:0046500 S-adenosylmethionine metabolic process

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.

[2] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[3] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.