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Overview

Uniprot ID	P23514
Protein Name	Coatomer subunit beta
Gene Name	Copb1
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
712	AADPLASKLNKVTQL

Function

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1. Plays a functional role in facilitating the transport of kappa-type opioid receptor mRNAs into axons and enhances translation of these proteins in the axonal compartment of dorsal root ganglion (DRG) cells. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis

Protein Sequence

10 MTAAENVCYT 20 LINVPMDSEP 30 PSEISLKNDL 40 EKGDVKSKTE 50 ALKKVIIMIL 60 NGEKLPGLLM 70 TIIRFVLPLQ 80 DHTIKKLLLV 90 FWEIVPKTTP 100 DGRLLHEMIL 110 VCDAYRKDLQ 120 HPNEFIRGST 130 LRFLCKLKEA 140 ELLEPLMPAI 150 RACLEHRHSY 160 VRRNAVLAIY 170 TIYRNFENLI 180 PDAPELIHDF 190 LVNEKDASCK 200 RNAFMMLIHA 210 DQDRALDYLS 220 TCIDQVQTFG 230 DILQLVIVEL 240 IYKVCHANPS 250 ERARFIRCIY 260 NLLQSSSPAV 270 KYEAAGTLVT 280 LSSAPTAIKA 290 AAQCYIDLII 300 KESDNNVKLI 310 VLDRLVELKE 320 HPAHERVLQD 330 LVMDILRVLS 340 TPDLEVRKKT 350 LQLALDLVSS 360 RNVEELVIVL 370 KKEVIKTNNV 380 SEHEDTDKYR 390 QLLVRTLHSC 400 SVRFPDMAAN 410 VIPVLMEFLS 420 DSNEAAAADV 430 LEFVREAIQR 440 FDNLRMLIVE 450 KMLEVFHAIK 460 SVKIYRGALW 470 ILGEYCSTKE 480 DIQSVMTEVR 490 RSLGEIPIVE 500 SEIKKEAGEL 510 KPEEEITVGP 520 VQKLVTEMGT 530 YATQSALSSS 540 RPTKKEEDRP 550 PLRGFLLDGD 560 FFVAASLATT 570 LTKIALRYVA 580 LVQEKKKQNS 590 FVAEAMLLMA 600 TILHLGKSSL 610 PKKPITDDDV 620 DRISLCLKVL 630 SECSPLMNDI 640 FNKECRQSLS 650 QMLSAKLEEE 660 KLSQKKESEK 670 RNVTVQPDDP 680 ISFMQLTAKN 690 EMNCKEDQFQ 700 LSLLAAMGNT 710 QRKEAADPLA 720 SKLNKVTQLT 730 GFSDPVYAEA 740 YVHVNQYDIV 750 LDVLVVNQTS 760 DTLQNCTLEL 770 ATLGDLKLVE 780 KPSPLTLAPH 790 DFANIKANVK 800 VASTENGIIF 810 GNIVYDVSGA 820 ASDRNCVVLS 830 DIHIDIMDYI 840 QPATCTDAEF 850 RQMWAEFEWE 860 NKVTVNTNVT 870 DLNDYLQHIL 880 KSTNMKCLTP 890 EKALSGYCGF 900 MAANLYARSI 910 FGEDALANVS 920 IEKPVHQGPD 930 AAVTGHIRIR 940 AKSQGMALSL 950 GDKINLSQKK TSL

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0030126	COPI vesicle coat
Cellular Component	GO:0030137	COPI-coated vesicle
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005793	endoplasmic reticulum-Golgi intermediate compartment
Cellular Component	GO:0005794	Golgi apparatus
Cellular Component	GO:0000139	Golgi membrane
Cellular Component	GO:0005798	Golgi-associated vesicle
Cellular Component	GO:0005886	plasma membrane
Molecular Function	GO:0005198	structural molecule activity
Biological Process	GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport
Biological Process	GO:0006891	intra-Golgi vesicle-mediated transport
Biological Process	GO:0006886	intracellular protein transport
Biological Process	GO:0006890	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.