Overview
| Uniprot ID | P23965 |
|---|
| Protein Name | Enoyl-CoA delta isomerase 1, mitochondrial |
|---|
| Gene Name | Eci1 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 255 |
ATADNLIKQREADIQ |
| 275 |
ISRDSIQKSLHVYLE |
| 283 |
SLHVYLEKLKQKKG* |
| 76 |
LEKLENDKSIRGVIL |
Function
Auxiliary enzyme in the beta-oxidation of mono- and polyunsaturated fatty acids (PubMed:11781327, PubMed:8218206). Together with the 2,4-dienoyl-CoA reductase (DECR) and the Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase (ECH1), they allow the reentrance of the enoyl-CoA into the beta-oxidation cycle. Able to isomerize both 3-cis (3Z) and 3-trans (3E) double bonds into the 2-trans (2E) form in a range of fatty enoyl-CoA species, with a preference for (3Z)-enoyl-CoAs over (3E)-enoyl-CoAs (PubMed:11781327, PubMed:8218206). The catalytic efficiency of this enzyme is not affected by the fatty acyl chain length, and based on its established catalytic mechanism, it is predicted to act also on other fatty acids besides those tested experimentally (Probable)
Protein Sequence
10
MALAAARRVL
20
LQAGSRLGRR
30
GAVDGARRFS
40
NKRVLVEKEG
50
EAGIAVMKFK
60
NPPVNSLSLE
70
FLTEFVISLE
80
KLENDKSIRG
90
VILTSERPGI
100
FSAGLDLMEM
110
YGRNPAHYAE
120
YWKAVQELWL
130
RLYLSNLTLI
140
SAINGASPAG
150
GCLMALTCDY
160
RIMADNSKYT
170
IGLNESLLGI
180
VAPFWLKDNY
190
VNTIGHRAAE
200
RALQLGTLFP
210
PAEALKVGLV
220
DEVVPEDQVH
230
SKARSVMAKW
240
FTIPDHSRQL
250
TKSMMRKATA
260
DNLIKQREAD
270
IQNFTSFISR
280
DSIQKSLHVY
LEKLKQKKG
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005759 |
mitochondrial matrix |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Molecular Function |
GO:0004165 |
delta(3)-delta(2)-enoyl-CoA isomerase activity |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0016863 |
intramolecular oxidoreductase activity, transposing C=C bonds |
| Biological Process |
GO:0006635 |
fatty acid beta-oxidation |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.