Search Results

Overview

Uniprot IDP26358
Protein NameDNA (cytosine-5)-methyltransferase 1
Gene NameDNMT1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1121 GKGKGKPKSQACEPS
142 PRTPRRSKSDGEAKP
173 TITSHFAKGPAKRKP
291 KKHRSQPKDLAAKRR
323 DEDEKEEKRRKTTPK
330 KRRKTTPKEPTEKKM
341 EKKMARAKTVMNSKT
347 AKTVMNSKTHPPKCI
961 SPVKRPRKEPVDEDL

Function

DNA methyltransferase that methylates CpG residues (PubMed:17200670, PubMed:18754681, PubMed:21745816, PubMed:26070743). Preferentially methylates hemimethylated DNA (PubMed:21745816, PubMed:26070743). Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance (PubMed:17200670, PubMed:21745816). Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication (PubMed:21745816). It is responsible for maintaining methylation patterns established in development (PubMed:21745816). DNA methylation is coordinated with methylation of histones (PubMed:16357870). Mediates transcriptional repression by direct binding to HDAC2 (PubMed:10888872). In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9 (PubMed:18413740). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306)

Protein Sequence

10 MPARTAPARV 20 PTLAVPAISL 30 PDDVRRRLKD 40 LERDSLTEKE 50 CVKEKLNLLH 60 EFLQTEIKNQ 70 LCDLETKLRK 80 EELSEEGYLA 90 KVKSLLNKDL 100 SLENGAHAYN 110 REVNGRLENG 120 NQARSEARRV 130 GMADANSPPK 140 PLSKPRTPRR 150 SKSDGEAKPE 160 PSPSPRITRK 170 STRQTTITSH 180 FAKGPAKRKP 190 QEESERAKSD 200 ESIKEEDKDQ 210 DEKRRRVTSR 220 ERVARPLPAE 230 EPERAKSGTR 240 TEKEEERDEK 250 EEKRLRSQTK 260 EPTPKQKLKE 270 EPDREARAGV 280 QADEDEDGDE 290 KDEKKHRSQP 300 KDLAAKRRPE 310 EKEPEKVNPQ 320 ISDEKDEDEK 330 EEKRRKTTPK 340 EPTEKKMARA 350 KTVMNSKTHP 360 PKCIQCGQYL 370 DDPDLKYGQH 380 PPDAVDEPQM 390 LTNEKLSIFD 400 ANESGFESYE 410 ALPQHKLTCF 420 SVYCKHGHLC 430 PIDTGLIEKN 440 IELFFSGSAK 450 PIYDDDPSLE 460 GGVNGKNLGP 470 INEWWITGFD 480 GGEKALIGFS 490 TSFAEYILMD 500 PSPEYAPIFG 510 LMQEKIYISK 520 IVVEFLQSNS 530 DSTYEDLINK 540 IETTVPPSGL 550 NLNRFTEDSL 560 LRHAQFVVEQ 570 VESYDEAGDS 580 DEQPIFLTPC 590 MRDLIKLAGV 600 TLGQRRAQAR 610 RQTIRHSTRE 620 KDRGPTKATT 630 TKLVYQIFDT 640 FFAEQIEKDD 650 REDKENAFKR 660 RRCGVCEVCQ 670 QPECGKCKAC 680 KDMVKFGGSG 690 RSKQACQERR 700 CPNMAMKEAD 710 DDEEVDDNIP 720 EMPSPKKMHQ 730 GKKKKQNKNR 740 ISWVGEAVKT 750 DGKKSYYKKV 760 CIDAETLEVG 770 DCVSVIPDDS 780 SKPLYLARVT 790 ALWEDSSNGQ 800 MFHAHWFCAG 810 TDTVLGATSD 820 PLELFLVDEC 830 EDMQLSYIHS 840 KVKVIYKAPS 850 ENWAMEGGMD 860 PESLLEGDDG 870 KTYFYQLWYD 880 QDYARFESPP 890 KTQPTEDNKF 900 KFCVSCARLA 910 EMRQKEIPRV 920 LEQLEDLDSR 930 VLYYSATKNG 940 ILYRVGDGVY 950 LPPEAFTFNI 960 KLSSPVKRPR 970 KEPVDEDLYP 980 EHYRKYSDYI 990 KGSNLDAPEP 1000 YRIGRIKEIF 1010 CPKKSNGRPN 1020 ETDIKIRVNK 1030 FYRPENTHKS 1040 TPASYHADIN 1050 LLYWSDEEAV 1060 VDFKAVQGRC 1070 TVEYGEDLPE 1080 CVQVYSMGGP 1090 NRFYFLEAYN 1100 AKSKSFEDPP 1110 NHARSPGNKG 1120 KGKGKGKGKP 1130 KSQACEPSEP 1140 EIEIKLPKLR 1150 TLDVFSGCGG 1160 LSEGFHQAGI 1170 SDTLWAIEMW 1180 DPAAQAFRLN 1190 NPGSTVFTED 1200 CNILLKLVMA 1210 GETTNSRGQR 1220 LPQKGDVEML 1230 CGGPPCQGFS 1240 GMNRFNSRTY 1250 SKFKNSLVVS 1260 FLSYCDYYRP 1270 RFFLLENVRN 1280 FVSFKRSMVL 1290 KLTLRCLVRM 1300 GYQCTFGVLQ 1310 AGQYGVAQTR 1320 RRAIILAAAP 1330 GEKLPLFPEP 1340 LHVFAPRACQ 1350 LSVVVDDKKF 1360 VSNITRLSSG 1370 PFRTITVRDT 1380 MSDLPEVRNG 1390 ASALEISYNG 1400 EPQSWFQRQL 1410 RGAQYQPILR 1420 DHICKDMSAL 1430 VAARMRHIPL 1440 APGSDWRDLP 1450 NIEVRLSDGT 1460 MARKLRYTHH 1470 DRKNGRSSSG 1480 ALRGVCSCVE 1490 AGKACDPAAR 1500 QFNTLIPWCL 1510 PHTGNRHNHW 1520 AGLYGRLEWD 1530 GFFSTTVTNP 1540 EPMGKQGRVL 1550 HPEQHRVVSV 1560 RECARSQGFP 1570 DTYRLFGNIL 1580 DKHRQVGNAV 1590 PPPLAKAIGL 1600 EIKLCMLAKA 1610 RESASAKIKE EEAAKD

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000792 heterochromatin
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005721 pericentric heterochromatin
Cellular Component GO:0005657 replication fork
Molecular Function GO:0003886 DNA (cytosine-5-)-methyltransferase activity
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0009008 DNA-methyltransferase activity
Molecular Function GO:0140258 histone H3K14ub reader activity
Molecular Function GO:0140254 histone H3K18ub reader activity
Molecular Function GO:0140257 histone H3K23ub reader activity
Molecular Function GO:0106222 lncRNA binding
Molecular Function GO:0008327 methyl-CpG binding
Molecular Function GO:1990841 promoter-specific chromatin binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0141119 chromosomal DNA methylation maintenance following DNA replication
Biological Process GO:0006346 DNA methylation-dependent constitutive heterochromatin formation
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0032259 methylation
Biological Process GO:0010629 negative regulation of gene expression
Biological Process GO:0044027 negative regulation of gene expression via chromosomal CpG island methylation
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:1905460 negative regulation of vascular associated smooth muscle cell apoptotic process
Biological Process GO:1905931 negative regulation of vascular associated smooth muscle cell differentiation involved in phenotypic switching
Biological Process GO:0010628 positive regulation of gene expression
Biological Process GO:1904707 positive regulation of vascular associated smooth muscle cell proliferation

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.