Search Results

Overview

Uniprot IDP27144
Protein NameAdenylate kinase 4, mitochondrial
Gene NameAK4
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
163 LVQQEDDKPEAVAAR
175 AARLRQYKDVAKPVI
179 RQYKDVAKPVIELYK
18 LGPPGSGKGTVCQRI
186 KPVIELYKSRGVLHQ
45 HFLRENIKASTEVGE
55 TEVGEMAKQYIEKSL
60 MAKQYIEKSLLVPDH

Function

Broad-specificity mitochondrial nucleoside phosphate kinase involved in cellular nucleotide homeostasis by catalyzing nucleoside-phosphate interconversions (PubMed:19073142, PubMed:19766732, PubMed:23416111, PubMed:24767988). Similar to other adenylate kinases, preferentially catalyzes the phosphorylation of the nucleoside monophosphate AMP with ATP as phosphate donor to produce ADP (PubMed:19766732). Phosphorylates only AMP when using GTP as phosphate donor (PubMed:19766732). In vitro, can also catalyze the phosphorylation of CMP, dAMP and dCMP and use GTP as an alternate phosphate donor (PubMed:19766732, PubMed:23416111). Moreover, exhibits a diphosphate kinase activity, producing ATP, CTP, GTP, UTP, TTP, dATP, dCTP and dGTP from the corresponding diphosphate substrates with either ATP or GTP as phosphate donors (PubMed:23416111). Plays a role in controlling cellular ATP levels by regulating phosphorylation and activation of the energy sensor protein kinase AMPK (PubMed:24767988, PubMed:26980435). Plays a protective role in the cellular response to oxidative stress (PubMed:19130895, PubMed:23474458, PubMed:26980435)

Protein Sequence

10 MASKLLRAVI 20 LGPPGSGKGT 30 VCQRIAQNFG 40 LQHLSSGHFL 50 RENIKASTEV 60 GEMAKQYIEK 70 SLLVPDHVIT 80 RLMMSELENR 90 RGQHWLLDGF 100 PRTLGQAEAL 110 DKICEVDLVI 120 SLNIPFETLK 130 DRLSRRWIHP 140 PSGRVYNLDF 150 NPPHVHGIDD 160 VTGEPLVQQE 170 DDKPEAVAAR 180 LRQYKDVAKP 190 VIELYKSRGV 200 LHQFSGTETN 210 KIWPYVYTLF 220 SNKITPIQSK EAY

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0004017 AMP kinase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0036430 CMP kinase activity
Molecular Function GO:0047506 dAMP kinase activity
Molecular Function GO:0036431 dCMP kinase activity
Molecular Function GO:0005525 GTP binding
Molecular Function GO:0004550 nucleoside diphosphate kinase activity
Molecular Function GO:0050145 nucleoside monophosphate kinase activity
Molecular Function GO:0046899 nucleoside triphosphate adenylate kinase activity
Biological Process GO:0006172 ADP biosynthetic process
Biological Process GO:0046033 AMP metabolic process
Biological Process GO:0046034 ATP metabolic process
Biological Process GO:0071456 cellular response to hypoxia
Biological Process GO:0046039 GTP metabolic process
Biological Process GO:0015949 nucleobase-containing small molecule interconversion
Biological Process GO:0009142 nucleoside triphosphate biosynthetic process
Biological Process GO:0002082 regulation of oxidative phosphorylation
Biological Process GO:0009188 ribonucleoside diphosphate biosynthetic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.