Search Results

Overview

Uniprot ID	P28331
Protein Name	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
Gene Name	NDUFS1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
311	EPMVRNEKGLLTYTS
420	LFNARIRKSWLHNDL
467	HPFSQVLKEAKKPMV
562	ITRQDLPKDCFIIYQ
84	MCLVEIEKAPKVVAA

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:30879903, PubMed:31557978). Essential for catalysing the entry and efficient transfer of electrons within complex I (PubMed:31557978). Plays a key role in the assembly and stability of complex I and participates in the association of complex I with ubiquinol-cytochrome reductase complex (Complex III) to form supercomplexes (PubMed:30879903, PubMed:31557978)

Protein Sequence

10 MLRIPVRKAL 20 VGLSKSPKGC 30 VRTTATAASN 40 LIEVFVDGQS 50 VMVEPGTTVL 60 QACEKVGMQI 70 PRFCYHERLS 80 VAGNCRMCLV 90 EIEKAPKVVA 100 ACAMPVMKGW 110 NILTNSEKSK 120 KAREGVMEFL 130 LANHPLDCPI 140 CDQGGECDLQ 150 DQSMMFGNDR 160 SRFLEGKRAV 170 EDKNIGPLVK 180 TIMTRCIQCT 190 RCIRFASEIA 200 GVDDLGTTGR 210 GNDMQVGTYI 220 EKMFMSELSG 230 NIIDICPVGA 240 LTSKPYAFTA 250 RPWETRKTES 260 IDVMDAVGSN 270 IVVSTRTGEV 280 MRILPRMHED 290 INEEWISDKT 300 RFAYDGLKRQ 310 RLTEPMVRNE 320 KGLLTYTSWE 330 DALSRVAGML 340 QSFQGKDVAA 350 IAGGLVDAEA 360 LVALKDLLNR 370 VDSDTLCTEE 380 VFPTAGAGTD 390 LRSNYLLNTT 400 IAGVEEADVV 410 LLVGTNPRFE 420 APLFNARIRK 430 SWLHNDLKVA 440 LIGSPVDLTY 450 TYDHLGDSPK 460 ILQDIASGSH 470 PFSQVLKEAK 480 KPMVVLGSSA 490 LQRNDGAAIL 500 AAVSSIAQKI 510 RMTSGVTGDW 520 KVMNILHRIA 530 SQVAALDLGY 540 KPGVEAIRKN 550 PPKVLFLLGA 560 DGGCITRQDL 570 PKDCFIIYQG 580 HHGDVGAPIA 590 DVILPGAAYT 600 EKSATYVNTE 610 GRAQQTKVAV 620 TPPGLAREDW 630 KIIRALSEIA 640 GMTLPYDTLD 650 QVRNRLEEVS 660 PNLVRYDDIE 670 GANYFQQANE 680 LSKLVNQQLL 690 ADPLVPPQLT 700 IKDFYMTDSI 710 SRASQTMAKC 720 VKAVTEGAQA VEEPSIC

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005743	mitochondrial inner membrane
Cellular Component	GO:0005758	mitochondrial intermembrane space
Cellular Component	GO:0005759	mitochondrial matrix
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0045271	respiratory chain complex I
Molecular Function	GO:0051537	2 iron, 2 sulfur cluster binding
Molecular Function	GO:0051539	4 iron, 4 sulfur cluster binding
Molecular Function	GO:0009055	electron transfer activity
Molecular Function	GO:0046872	metal ion binding
Molecular Function	GO:0008137	NADH dehydrogenase (ubiquinone) activity
Molecular Function	GO:0016651	oxidoreductase activity, acting on NAD(P)H
Biological Process	GO:0009060	aerobic respiration
Biological Process	GO:0045333	cellular respiration
Biological Process	GO:0006120	mitochondrial electron transport, NADH to ubiquinone
Biological Process	GO:0032981	mitochondrial respiratory chain complex I assembly
Biological Process	GO:0042776	proton motive force-driven mitochondrial ATP synthesis

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.