Search Results

Overview

Uniprot IDP28340
Protein NameDNA polymerase delta catalytic subunit
Gene NamePOLD1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
439 RDSSFQSKQTGRRDT
738 TKQLVESKYTVENGY
897 AASDYAGKQAHVELA
998 CKTVLTGKVGGLLAF

Function

As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24022480, PubMed:24035200, PubMed:31449058). Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24022480, PubMed:24035200). Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine, 8oxoG or abasic sites (PubMed:19074196, PubMed:24191025)

Protein Sequence

10 MDGKRRPGPG 20 PGVPPKRARG 30 GLWDDDDAPR 40 PSQFEEDLAL 50 MEEMEAEHRL 60 QEQEEEELQS 70 VLEGVADGQV 80 PPSAIDPRWL 90 RPTPPALDPQ 100 TEPLIFQQLE 110 IDHYVGPAQP 120 VPGGPPPSRG 130 SVPVLRAFGV 140 TDEGFSVCCH 150 IHGFAPYFYT 160 PAPPGFGPEH 170 MGDLQRELNL 180 AISRDSRGGR 190 ELTGPAVLAV 200 ELCSRESMFG 210 YHGHGPSPFL 220 RITVALPRLV 230 APARRLLEQG 240 IRVAGLGTPS 250 FAPYEANVDF 260 EIRFMVDTDI 270 VGCNWLELPA 280 GKYALRLKEK 290 ATQCQLEADV 300 LWSDVVSHPP 310 EGPWQRIAPL 320 RVLSFDIECA 330 GRKGIFPEPE 340 RDPVIQICSL 350 GLRWGEPEPF 360 LRLALTLRPC 370 APILGAKVQS 380 YEKEEDLLQA 390 WSTFIRIMDP 400 DVITGYNIQN 410 FDLPYLISRA 420 QTLKVQTFPF 430 LGRVAGLCSN 440 IRDSSFQSKQ 450 TGRRDTKVVS 460 MVGRVQMDML 470 QVLLREYKLR 480 SYTLNAVSFH 490 FLGEQKEDVQ 500 HSIITDLQNG 510 NDQTRRRLAV 520 YCLKDAYLPL 530 RLLERLMVLV 540 NAVEMARVTG 550 VPLSYLLSRG 560 QQVKVVSQLL 570 RQAMHEGLLM 580 PVVKSEGGED 590 YTGATVIEPL 600 KGYYDVPIAT 610 LDFSSLYPSI 620 MMAHNLCYTT 630 LLRPGTAQKL 640 GLTEDQFIRT 650 PTGDEFVKTS 660 VRKGLLPQIL 670 ENLLSARKRA 680 KAELAKETDP 690 LRRQVLDGRQ 700 LALKVSANSV 710 YGFTGAQVGK 720 LPCLEISQSV 730 TGFGRQMIEK 740 TKQLVESKYT 750 VENGYSTSAK 760 VVYGDTDSVM 770 CRFGVSSVAE 780 AMALGREAAD 790 WVSGHFPSPI 800 RLEFEKVYFP 810 YLLISKKRYA 820 GLLFSSRPDA 830 HDRMDCKGLE 840 AVRRDNCPLV 850 ANLVTASLRR 860 LLIDRDPEGA 870 VAHAQDVISD 880 LLCNRIDISQ 890 LVITKELTRA 900 ASDYAGKQAH 910 VELAERMRKR 920 DPGSAPSLGD 930 RVPYVIISAA 940 KGVAAYMKSE 950 DPLFVLEHSL 960 PIDTQYYLEQ 970 QLAKPLLRIF 980 EPILGEGRAE 990 AVLLRGDHTR 1000 CKTVLTGKVG 1010 GLLAFAKRRN 1020 CCIGCRTVLS 1030 HQGAVCEFCQ 1040 PRESELYQKE 1050 VSHLNALEER 1060 FSRLWTQCQR 1070 CQGSLHEDVI 1080 CTSRDCPIFY 1090 MRKKVRKDLE 1100 DQEQLLRRFG PPGPEAW

Gene Ontology

Classification GO ID Description
Biological Process GO:0070987 error-free translesion synthesis
Cellular Component GO:0043625 delta DNA polymerase complex
Cellular Component GO:0016020 membrane
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0000109 nucleotide-excision repair complex
Cellular Component GO:0005634 nucleus
Molecular Function GO:0008296 3'-5'-DNA exonuclease activity
Molecular Function GO:0051539 4 iron, 4 sulfur cluster binding
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0003684 damaged DNA binding
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0003887 DNA-directed DNA polymerase activity
Molecular Function GO:0019899 enzyme binding
Molecular Function GO:0000166 nucleotide binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006287 base-excision repair, gap-filling
Biological Process GO:0071897 DNA biosynthetic process
Biological Process GO:0006281 DNA repair
Biological Process GO:0006260 DNA replication
Biological Process GO:0045004 DNA replication proofreading
Biological Process GO:0000731 DNA synthesis involved in DNA repair
Biological Process GO:0006261 DNA-templated DNA replication
Biological Process GO:0055089 fatty acid homeostasis
Biological Process GO:0006297 nucleotide-excision repair, DNA gap filling
Biological Process GO:0009411 response to UV

Reference

[1] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[2] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.