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Overview

Uniprot IDP28715
Protein NameDNA excision repair protein ERCC-5
Gene NameERCC5
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1071 LEESSSLKRKRLSDS
235 DFSQYQLKGLLKKNY
293 TSHYILIKGIQAKTV
313 ESLPSSSKMHGMSFD

Function

Single-stranded structure-specific DNA endonuclease involved in DNA excision repair (PubMed:32522879, PubMed:32821917, PubMed:7651464, PubMed:8078765, PubMed:8090225, PubMed:8206890). Makes the 3'incision in DNA nucleotide excision repair (NER) (PubMed:32522879, PubMed:32821917, PubMed:8078765, PubMed:8090225). Binds and bends DNA repair bubble substrate and breaks base stacking at the single-strand/double-strand DNA junction of the DNA bubble (PubMed:32522879). Plays a role in base excision repair (BER) by promoting the binding of DNA glycosylase NTHL1 to its substrate and increasing NTHL1 catalytic activity that removes oxidized pyrimidines from DNA (PubMed:9927729). Involved in transcription-coupled nucleotide excision repair (TCR) which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes (PubMed:16246722). Functions during the initial step of TCR in cooperation with ERCC6/CSB to recognized stalled RNA polymerase II (PubMed:16246722). Also, stimulates ERCC6/CSB binding to the DNA repair bubble and ERCC6/CSB ATPase activity (PubMed:16246722). Required for DNA replication fork maintenance and preservation of genomic stability (PubMed:26833090, PubMed:32522879). Involved in homologous recombination repair (HRR) induced by DNA replication stress by recruiting RAD51, BRCA2, and PALB2 to the damaged DNA site (PubMed:26833090). In TFIIH stimulates the 5'-3' helicase activity of XPD/ERCC2 and the DNA translocase activity of XPB/ERCC3 (PubMed:31253769). During HRR, binds to the replication fork with high specificity and stabilizes it (PubMed:32522879). Also, acts upstream of HRR, to promote the release of BRCA1 from DNA (PubMed:26833090)

Protein Sequence

10 MGVQGLWKLL 20 ECSGRQVSPE 30 ALEGKILAVD 40 ISIWLNQALK 50 GVRDRHGNSI 60 ENPHLLTLFH 70 RLCKLLFFRI 80 RPIFVFDGDA 90 PLLKKQTLVK 100 RRQRKDLASS 110 DSRKTTEKLL 120 KTFLKRQAIK 130 TAFRSKRDEA 140 LPSLTQVRRE 150 NDLYVLPPLQ 160 EEEKHSSEEE 170 DEKEWQERMN 180 QKQALQEEFF 190 HNPQAIDIES 200 EDFSSLPPEV 210 KHEILTDMKE 220 FTKRRRTLFE 230 AMPEESDDFS 240 QYQLKGLLKK 250 NYLNQHIEHV 260 QKEMNQQHSG 270 HIRRQYEDEG 280 GFLKEVESRR 290 VVSEDTSHYI 300 LIKGIQAKTV 310 AEVDSESLPS 320 SSKMHGMSFD 330 VKSSPCEKLK 340 TEKEPDATPP 350 SPRTLLAMQA 360 ALLGSSSEEE 370 LESENRRQAR 380 GRNAPAAVDE 390 GSISPRTLSA 400 IKRALDDDED 410 VKVCAGDDVQ 420 TGGPGAEEMR 430 INSSTENSDE 440 GLKVRDGKGI 450 PFTATLASSS 460 VNSAEEHVAS 470 TNEGREPTDS 480 VPKEQMSLVH 490 VGTEAFPISD 500 ESMIKDRKDR 510 LPLESAVVRH 520 SDAPGLPNGR 530 ELTPASPTCT 540 NSVSKNETHA 550 EVLEQQNELC 560 PYESKFDSSL 570 LSSDDETKCK 580 PNSASEVIGP 590 VSLQETSSIV 600 SVPSEAVDNV 610 ENVVSFNAKE 620 HENFLETIQE 630 QQTTESAGQD 640 LISIPKAVEP 650 MEIDSEESES 660 DGSFIEVQSV 670 ISDEELQAEF 680 PETSKPPSEQ 690 GEEELVGTRE 700 GEAPAESESL 710 LRDNSERDDV 720 DGEPQEAEKD 730 AEDSLHEWQD 740 INLEELETLE 750 SNLLAQQNSL 760 KAQKQQQERI 770 AATVTGQMFL 780 ESQELLRLFG 790 IPYIQAPMEA 800 EAQCAILDLT 810 DQTSGTITDD 820 SDIWLFGARH 830 VYRNFFNKNK 840 FVEYYQYVDF 850 HNQLGLDRNK 860 LINLAYLLGS 870 DYTEGIPTVG 880 CVTAMEILNE 890 FPGHGLEPLL 900 KFSEWWHEAQ 910 KNPKIRPNPH 920 DTKVKKKLRT 930 LQLTPGFPNP 940 AVAEAYLKPV 950 VDDSKGSFLW 960 GKPDLDKIRE 970 FCQRYFGWNR 980 TKTDESLFPV 990 LKQLDAQQTQ 1000 LRIDSFFRLA 1010 QQEKEDAKRI 1020 KSQRLNRAVT 1030 CMLRKEKEAA 1040 ASEIEAVSVA 1050 MEKEFELLDK 1060 AKGKTQKRGI 1070 TNTLEESSSL 1080 KRKRLSDSKG 1090 KNTCGGFLGE 1100 TCLSESSDGS 1110 SSEDAESSSL 1120 MNVQRRTAAK 1130 EPKTSASDSQ 1140 NSVKEAPVKN 1150 GGATTSSSSD 1160 SDDDGGKEKM 1170 VLVTARSVFG 1180 KKRRKLRRAR GRKRKT

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005694 chromosome
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0000109 nucleotide-excision repair complex
Cellular Component GO:0005634 nucleus
Cellular Component GO:0032991 protein-containing complex
Molecular Function GO:0000405 bubble DNA binding
Molecular Function GO:0003684 damaged DNA binding
Molecular Function GO:0004520 DNA endonuclease activity
Molecular Function GO:0003690 double-stranded DNA binding
Molecular Function GO:0004519 endonuclease activity
Molecular Function GO:0008047 enzyme activator activity
Molecular Function GO:0016788 hydrolase activity, acting on ester bonds
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0044877 protein-containing complex binding
Molecular Function GO:0000993 RNA polymerase II complex binding
Molecular Function GO:0003697 single-stranded DNA binding
Biological Process GO:0006285 base-excision repair, AP site formation
Biological Process GO:0000724 double-strand break repair via homologous recombination
Biological Process GO:0043066 negative regulation of apoptotic process
Biological Process GO:0006289 nucleotide-excision repair
Biological Process GO:0009411 response to UV
Biological Process GO:0010225 response to UV-C
Biological Process GO:0006283 transcription-coupled nucleotide-excision repair

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.