Search Results

Overview

Uniprot IDP30041
Protein NamePeroxiredoxin-6
Gene NamePRDX6
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
122 GMLDPAEKDEKGMPV
125 DPAEKDEKGMPVTAR
141 VFVFGPDKKLKLSIL
142 FVFGPDKKLKLSILY
144 FGPDKKLKLSILYPA
204 EAKKLFPKGVFTKEL
209 FPKGVFTKELPSGKK
215 TKELPSGKKYLRYTP
216 KELPSGKKYLRYTPQ
56 TELGRAAKLAPEFAK
63 KLAPEFAKRNVKLIA
84 EDHLAWSKDINAYNC

Function

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively (PubMed:10893423, PubMed:9497358). Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides (PubMed:10893423). Also has phospholipase activity, can therefore either reduce the oxidized sn-2 fatty acyl group of phospholipids (peroxidase activity) or hydrolyze the sn-2 ester bond of phospholipids (phospholipase activity) (PubMed:10893423, PubMed:26830860). These activities are dependent on binding to phospholipids at acidic pH and to oxidized phospholipds at cytosolic pH (PubMed:10893423). Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis (PubMed:10893423). Exhibits acyl-CoA-dependent lysophospholipid acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (PubMed:26830860). Shows a clear preference for LPC as the lysophospholipid and for palmitoyl CoA as the fatty acyl substrate (PubMed:26830860)

Protein Sequence

10 MPGGLLLGDV 20 APNFEANTTV 30 GRIRFHDFLG 40 DSWGILFSHP 50 RDFTPVCTTE 60 LGRAAKLAPE 70 FAKRNVKLIA 80 LSIDSVEDHL 90 AWSKDINAYN 100 CEEPTEKLPF 110 PIIDDRNREL 120 AILLGMLDPA 130 EKDEKGMPVT 140 ARVVFVFGPD 150 KKLKLSILYP 160 ATTGRNFDEI 170 LRVVISLQLT 180 AEKRVATPVD 190 WKDGDSVMVL 200 PTIPEEEAKK 210 LFPKGVFTKE 220 LPSGKKYLRY TPQP

Gene Ontology

Classification GO ID Description
Cellular Component GO:0035578 azurophil granule lumen
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005576 extracellular region
Cellular Component GO:0005615 extracellular space
Cellular Component GO:0016020 membrane
Cellular Component GO:0005634 nucleus
Cellular Component GO:0048471 perinuclear region of cytoplasm
Molecular Function GO:0047184 1-acylglycerophosphocholine O-acyltransferase activity
Molecular Function GO:0004623 A2-type glycerophospholipase activity
Molecular Function GO:0045296 cadherin binding
Molecular Function GO:0004602 glutathione peroxidase activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0004601 peroxidase activity
Molecular Function GO:0051920 peroxiredoxin activity
Molecular Function GO:0031625 ubiquitin protein ligase binding
Biological Process GO:0045454 cell redox homeostasis
Biological Process GO:0098869 cellular oxidant detoxification
Biological Process GO:0046475 glycerophospholipid catabolic process
Biological Process GO:0042744 hydrogen peroxide catabolic process
Biological Process GO:0048026 positive regulation of mRNA splicing, via spliceosome
Biological Process GO:0006979 response to oxidative stress

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[5] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[6] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[7] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[8] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[9] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.