Search Results

Overview

Uniprot ID	P30419
Protein Name	Glycylpeptide N-tetradecanoyltransferase 1
Gene Name	NMT1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
55	PANDTGAKKKKKKQK
56	ANDTGAKKKKKKQKK
65	KKKQKKKKEKGSETD
67	KQKKKKEKGSETDSA

Function

Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins (PubMed:22865860, PubMed:25255805, PubMed:32686708, PubMed:34999170, PubMed:9353336, PubMed:9506952). Also able to mediate N-terminal lysine myristoylation of proteins: catalyzes myristoylation of ARF6 on both 'Gly-2' and 'Lys-3' (PubMed:32103017, PubMed:32111831). Lysine myristoylation is required to maintain ARF6 on membranes during the GTPase cycle (PubMed:32103017)

Protein Sequence

10 MADESETAVK 20 PPAPPLPQMM 30 EGNGNGHEHC 40 SDCENEEDNS 50 YNRGGLSPAN 60 DTGAKKKKKK 70 QKKKKEKGSE 80 TDSAQDQPVK 90 MNSLPAERIQ 100 EIQKAIELFS 110 VGQGPAKTME 120 EASKRSYQFW 130 DTQPVPKLGE 140 VVNTHGPVEP 150 DKDNIRQEPY 160 TLPQGFTWDA 170 LDLGDRGVLK 180 ELYTLLNENY 190 VEDDDNMFRF 200 DYSPEFLLWA 210 LRPPGWLPQW 220 HCGVRVVSSR 230 KLVGFISAIP 240 ANIHIYDTEK 250 KMVEINFLCV 260 HKKLRSKRVA 270 PVLIREITRR 280 VHLEGIFQAV 290 YTAGVVLPKP 300 VGTCRYWHRS 310 LNPRKLIEVK 320 FSHLSRNMTM 330 QRTMKLYRLP 340 ETPKTAGLRP 350 METKDIPVVH 360 QLLTRYLKQF 370 HLTPVMSQEE 380 VEHWFYPQEN 390 IIDTFVVENA 400 NGEVTDFLSF 410 YTLPSTIMNH 420 PTHKSLKAAY 430 SFYNVHTQTP 440 LLDLMSDALV 450 LAKMKGFDVF 460 NALDLMENKT 470 FLEKLKFGIG 480 DGNLQYYLYN 490 WKCPSMGAEK VGLVLQ

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005886	plasma membrane
Molecular Function	GO:0004379	glycylpeptide N-tetradecanoyltransferase activity
Molecular Function	GO:0019107	myristoyltransferase activity
Molecular Function	GO:0018030	peptidyl-lysine N6-myristoyltransferase activity
Biological Process	GO:0042180	ketone metabolic process
Biological Process	GO:0018008	N-terminal peptidyl-glycine N-myristoylation
Biological Process	GO:0006499	N-terminal protein myristoylation
Biological Process	GO:1903749	positive regulation of establishment of protein localization to mitochondrion
Biological Process	GO:0072657	protein localization to membrane
Biological Process	GO:0022400	regulation of opsin-mediated signaling pathway

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.