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Overview

Uniprot IDP30613
Protein NamePyruvate kinase PKLR
Gene NamePKLR
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
109 ERLKEMIKAGMNIAR
290 VFASFVRKASDVAAV
309 GPEGHGIKIISKIEN
313 HGIKIISKIENHEGV
321 IENHEGVKRFDEILE
419 NFPVEAVKMQHAIAR
541 QFGIESGKLRGFLRV

Function

Pyruvate kinase that catalyzes the conversion of phosphoenolpyruvate to pyruvate with the synthesis of ATP, and which plays a key role in glycolysis (PubMed:11960989). Also produces the side product 2-phospholactate which can inhibit fructose-2,6-bisphosphate production (PubMed:27294321). 2-phospholactate can be dephosphorylated by PGP which prevents the inhibition of fructose-2,6-bisphosphate production and allows glycolysis to occur (PubMed:27294321)

Protein Sequence

10 MSIQENISSL 20 QLRSWVSKSQ 30 RDLAKSILIG 40 APGGPAGYLR 50 RASVAQLTQE 60 LGTAFFQQQQ 70 LPAAMADTFL 80 EHLCLLDIDS 90 EPVAARSTSI 100 IATIGPASRS 110 VERLKEMIKA 120 GMNIARLNFS 130 HGSHEYHAES 140 IANVREAVES 150 FAGSPLSYRP 160 VAIALDTKGP 170 EIRTGILQGG 180 PESEVELVKG 190 SQVLVTVDPA 200 FRTRGNANTV 210 WVDYPNIVRV 220 VPVGGRIYID 230 DGLISLVVQK 240 IGPEGLVTQV 250 ENGGVLGSRK 260 GVNLPGAQVD 270 LPGLSEQDVR 280 DLRFGVEHGV 290 DIVFASFVRK 300 ASDVAAVRAA 310 LGPEGHGIKI 320 ISKIENHEGV 330 KRFDEILEVS 340 DGIMVARGDL 350 GIEIPAEKVF 360 LAQKMMIGRC 370 NLAGKPVVCA 380 TQMLESMITK 390 PRPTRAETSD 400 VANAVLDGAD 410 CIMLSGETAK 420 GNFPVEAVKM 430 QHAIAREAEA 440 AVYHRQLFEE 450 LRRAAPLSRD 460 PTEVTAIGAV 470 EAAFKCCAAA 480 IIVLTTTGRS 490 AQLLSRYRPR 500 AAVIAVTRSA 510 QAARQVHLCR 520 GVFPLLYREP 530 PEAIWADDVD 540 RRVQFGIESG 550 KLRGFLRVGD 560 LVIVVTGWRP 570 GSGYTNIMRV LSIS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016301 kinase activity
Molecular Function GO:0000287 magnesium ion binding
Molecular Function GO:0048029 monosaccharide binding
Molecular Function GO:0030955 potassium ion binding
Molecular Function GO:0004743 pyruvate kinase activity
Biological Process GO:0071872 cellular response to epinephrine stimulus
Biological Process GO:0032869 cellular response to insulin stimulus
Biological Process GO:0006096 glycolytic process
Biological Process GO:0042866 pyruvate biosynthetic process
Biological Process GO:0033198 response to ATP
Biological Process GO:0051591 response to cAMP
Biological Process GO:0009749 response to glucose
Biological Process GO:0001666 response to hypoxia
Biological Process GO:0010038 response to metal ion
Biological Process GO:0007584 response to nutrient

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.