Search Results

Overview

Uniprot IDP30740
Protein NameLeukocyte elastase inhibitor
Gene NameSERPINB1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
177 NWKDKFMKEATTNAP
203 KMMYQKKKFAYGYIE
317 ARDIFISKIVHKSFV
56 NTAAQLSKTFHFNTV
79 SLNADINKRGASYIL

Function

Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis (PubMed:30692621). Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3 (PubMed:11747453, PubMed:30692621). Also acts as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity (PubMed:23269243). During inflammation, limits the activity of inflammatory caspases CASP1, CASP4 and CASP5 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation (PubMed:30692621). When secreted, promotes the proliferation of beta-cells via its protease inhibitory function (PubMed:26701651)

Protein Sequence

10 MEQLSSANTR 20 FALDLFLALS 30 ENNPAGNIFI 40 SPFSISSAMA 50 MVFLGTRGNT 60 AAQLSKTFHF 70 NTVEEVHSRF 80 QSLNADINKR 90 GASYILKLAN 100 RLYGEKTYNF 110 LPEFLVSTQK 120 TYGADLASVD 130 FQHASEDARK 140 TINQWVKGQT 150 EGKIPELLAS 160 GMVDNMTKLV 170 LVNAIYFKGN 180 WKDKFMKEAT 190 TNAPFRLNKK 200 DRKTVKMMYQ 210 KKKFAYGYIE 220 DLKCRVLELP 230 YQGEELSMVI 240 LLPDDIEDES 250 TGLKKIEEQL 260 TLEKLHEWTK 270 PENLDFIEVN 280 VSLPRFKLEE 290 SYTLNSDLAR 300 LGVQDLFNSS 310 KADLSGMSGA 320 RDIFISKIVH 330 KSFVEVNEEG 340 TEAAAATAGI 350 ATFCMLMPEE 360 NFTADHPFLF 370 FIRHNSSGSI LFLGRFSSP

Gene Ontology

Classification GO ID Description
Cellular Component GO:0044194 cytolytic granule
Cellular Component GO:0036464 cytoplasmic ribonucleoprotein granule
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005769 early endosome
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0031012 extracellular matrix
Cellular Component GO:0005576 extracellular region
Cellular Component GO:0005615 extracellular space
Cellular Component GO:0016020 membrane
Cellular Component GO:0034774 secretory granule lumen
Molecular Function GO:0030414 peptidase inhibitor activity
Molecular Function GO:0004867 serine-type endopeptidase inhibitor activity
Biological Process GO:0030336 negative regulation of cell migration
Biological Process GO:0010951 negative regulation of endopeptidase activity
Biological Process GO:0032691 negative regulation of interleukin-1 beta production
Biological Process GO:0044342 type B pancreatic cell proliferation

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.