Search Results

Overview

Uniprot ID	P30835
Protein Name	ATP-dependent 6-phosphofructokinase, liver type
Gene Name	Pfkl
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
386	ENNWKIYKLLAHQKV
392	YKLLAHQKVSKEKSN
677	FDRNYGTKLGVKAML
727	SSVTELKKETDFEHR

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (By similarity). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (By similarity)

Protein Sequence

10 MATVDLEKLR 20 MSGAGKAIGV 30 LTSGGDAQGM 40 NAAVRAVTRM 50 GIYVGAKVFL 60 IYEGYEGLVE 70 GGENIKPANW 80 LSVSNIIQLG 90 GTIIGSARCK 100 AFTTREGRLA 110 AAYNLLQHGI 120 TNLCVIGGDG 130 SLTGANIFRN 140 EWGSLLEELV 150 KEGKISESTA 160 QNYAHLSIAG 170 LVGSIDNDFC 180 GTDMTIGTDS 190 ALHRIMEVID 200 AITTTAQSHQ 210 RTFVLEVMGR 220 HCGYLALVSA 230 LASGADWLFI 240 PEAPPEDGWE 250 NFMCERLGET 260 RSRGSRLNII 270 IIAEGAIDRH 280 GKPISSSYVK 290 DLVVQRLGFD 300 TRVTVLGHVQ 310 RGGTPSAFDR 320 VLSSKMGMEA 330 VMALLEATPD 340 TPACVVSLSG 350 NQSVRLPLME 360 CVQVTKDVQK 370 AMDEKRFDEA 380 IQLRGRSFEN 390 NWKIYKLLAH 400 QKVSKEKSNF 410 SLAILNVGAP 420 AAGMNAAVRS 430 AVRTGISEGH 440 TVYVVHDGFE 450 GLAKGQVQEV 460 GWHDVAGWLG 470 RGGSMLGTKR 480 TLPKPHLEAI 490 VENLRTYNIH 500 ALLVIGGFEA 510 YEGVLQLVEA 520 RGRYEELCIV 530 MCVIPATISN 540 NVPGTDFSLG 550 SDTAVNAAME 560 SCDRIKQSAS 570 GTKRRVFIVE 580 TMGGYCGYLA 590 TVTGIAVGAD 600 AAYVFEDPFN 610 IHDLKANVEH 620 MTEKMKTDIQ 630 RGLVLRNEKC 640 HEHYTTEFLY 650 NLYSSEGRGV 660 FDCRTNVLGH 670 LQQGGAPTPF 680 DRNYGTKLGV 690 KAMLWMSEKL 700 RDVYRKGRVF 710 ANAPDSACVI 720 GLRKKVVAFS 730 SVTELKKETD 740 FEHRMPREQW 750 WLNLRLMLKM 760 LAHYRISMAD 770 YVSGELEHVT 780 RRTLSIDKGF

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005945	6-phosphofructokinase complex
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0016020	membrane
Molecular Function	GO:0003872	6-phosphofructokinase activity
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0070061	fructose binding
Molecular Function	GO:0004331	fructose-2,6-bisphosphate 2-phosphatase activity
Molecular Function	GO:0070095	fructose-6-phosphate binding
Molecular Function	GO:0042802	identical protein binding
Molecular Function	GO:0019900	kinase binding
Molecular Function	GO:0046872	metal ion binding
Molecular Function	GO:0048029	monosaccharide binding
Biological Process	GO:0061621	canonical glycolysis
Biological Process	GO:0030388	fructose 1,6-bisphosphate metabolic process
Biological Process	GO:0006002	fructose 6-phosphate metabolic process
Biological Process	GO:0006096	glycolytic process
Biological Process	GO:0061615	glycolytic process through fructose-6-phosphate
Biological Process	GO:0046676	negative regulation of insulin secretion
Biological Process	GO:0009749	response to glucose

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[3] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.