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Overview

Uniprot IDP32456
Protein NameGuanylate-binding protein 2
Gene NameGBP2
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
215 KGTDKKSKSFNDPRL
381 VDQMFQRKLGAQLEA
444 KLQELKNKYYQVPRK
451 KYYQVPRKGIQAKEV
456 PRKGIQAKEVLKKYL

Function

Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens (PubMed:31091448). Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions, but the major reaction product is GDP (PubMed:8706832). Following infection, recruited to the pathogen-containing vacuoles or vacuole-escaped bacteria and acts as a positive regulator of inflammasome assembly by promoting the release of inflammasome ligands from bacteria (By similarity). Acts by promoting lysis of pathogen-containing vacuoles, releasing pathogens into the cytosol (By similarity). Following pathogen release in the cytosol, promotes recruitment of proteins that mediate bacterial cytolysis: this liberates ligands that are detected by inflammasomes, such as lipopolysaccharide (LPS) that activates the non-canonical CASP4/CASP11 inflammasome or double-stranded DNA (dsDNA) that activates the AIM2 inflammasome (By similarity). Confers protection to the protozoan pathogen Toxoplasma gondii (By similarity). Independently of its GTPase activity, acts as an inhibitor of various viruses infectivity, such as HIV-1, Zika and influenza A viruses, by inhibiting FURIN-mediated maturation of viral envelope proteins (PubMed:31091448)

Protein Sequence

10 MAPEINLPGP 20 MSLIDNTKGQ 30 LVVNPEALKI 40 LSAITQPVVV 50 VAIVGLYRTG 60 KSYLMNKLAG 70 KKNGFSLGST 80 VKSHTKGIWM 90 WCVPHPKKPE 100 HTLVLLDTEG 110 LGDIEKGDNE 120 NDSWIFALAI 130 LLSSTFVYNS 140 MGTINQQAMD 150 QLHYVTELTD 160 RIKANSSPGN 170 NSVDDSADFV 180 SFFPAFVWTL 190 RDFTLELEVD 200 GEPITADDYL 210 ELSLKLRKGT 220 DKKSKSFNDP 230 RLCIRKFFPK 240 RKCFVFDWPA 250 PKKYLAHLEQ 260 LKEEELNPDF 270 IEQVAEFCSY 280 ILSHSNVKTL 290 SGGIPVNGPR 300 LESLVLTYVN 310 AISSGDLPCM 320 ENAVLALAQI 330 ENSAAVEKAI 340 AHYEQQMGQK 350 VQLPTETLQE 360 LLDLHRDSER 370 EAIEVFMKNS 380 FKDVDQMFQR 390 KLGAQLEARR 400 DDFCKQNSKA 410 SSDCCMALLQ 420 DIFGPLEEDV 430 KQGTFSKPGG 440 YRLFTQKLQE 450 LKNKYYQVPR 460 KGIQAKEVLK 470 KYLESKEDVA 480 DALLQTDQSL 490 SEKEKAIEVE 500 RIKAESAEAA 510 KKMLEEIQKK 520 NEEMMEQKEK 530 SYQEHVKQLT 540 EKMERDRAQL 550 MAEQEKTLAL 560 KLQEQERLLK 570 EGFENESKRL 580 QKDIWDIQMR 590 SKSLEPICNI L

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0031410 cytoplasmic vesicle
Cellular Component GO:0030659 cytoplasmic vesicle membrane
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005794 Golgi apparatus
Cellular Component GO:0000139 Golgi membrane
Cellular Component GO:0005634 nucleus
Cellular Component GO:0048471 perinuclear region of cytoplasm
Molecular Function GO:0005525 GTP binding
Molecular Function GO:0003924 GTPase activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0140678 molecular function inhibitor activity
Molecular Function GO:0042803 protein homodimerization activity
Biological Process GO:0002218 activation of innate immune response
Biological Process GO:0071347 cellular response to interleukin-1
Biological Process GO:0071222 cellular response to lipopolysaccharide
Biological Process GO:0071356 cellular response to tumor necrosis factor
Biological Process GO:0071346 cellular response to type II interferon
Biological Process GO:0051715 cytolysis in another organism
Biological Process GO:0042742 defense response to bacterium
Biological Process GO:0050830 defense response to Gram-positive bacterium
Biological Process GO:0042832 defense response to protozoan
Biological Process GO:0051607 defense response to virus
Biological Process GO:0006955 immune response
Biological Process GO:0140973 positive regulation of AIM2 inflammasome complex assembly
Biological Process GO:0140639 positive regulation of pyroptotic inflammatory response
Biological Process GO:0034504 protein localization to nucleus

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.