Overview
| Uniprot ID | P33991 |
|---|
| Protein Name | DNA replication licensing factor MCM4 |
|---|
| Gene Name | MCM4 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 858 |
DFLTVTGKTVRLL** |
Function
Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:16899510, PubMed:25661590, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232, PubMed:9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:16899510, PubMed:25661590, PubMed:32453425, PubMed:9305914)
Protein Sequence
10
MSSPASTPSR
20
RGSRRGRATP
30
AQTPRSEDAR
40
SSPSQRRRGE
50
DSTSTGELQP
60
MPTSPGVDLQ
70
SPAAQDVLFS
80
SPPQMHSSAI
90
PLDFDVSSPL
100
TYGTPSSRVE
110
GTPRSGVRGT
120
PVRQRPDLGS
130
AQKGLQVDLQ
140
SDGAAAEDIV
150
ASEQSLGQKL
160
VIWGTDVNVA
170
ACKENFQRFL
180
QRFIDPLAKE
190
EENVGIDITE
200
PLYMQRLGEI
210
NVIGEPFLNV
220
NCEHIKSFDK
230
NLYRQLISYP
240
QEVIPTFDMA
250
VNEIFFDRYP
260
DSILEHQIQV
270
RPFNALKTKN
280
MRNLNPEDID
290
QLITISGMVI
300
RTSQLIPEMQ
310
EAFFQCQVCA
320
HTTRVEMDRG
330
RIAEPSVCGR
340
CHTTHSMALI
350
HNRSLFSDKQ
360
MIKLQESPED
370
MPAGQTPHTV
380
ILFAHNDLVD
390
KVQPGDRVNV
400
TGIYRAVPIR
410
VNPRVSNVKS
420
VYKTHIDVIH
430
YRKTDAKRLH
440
GLDEEAEQKL
450
FSEKRVELLK
460
ELSRKPDIYE
470
RLASALAPSI
480
YEHEDIKKGI
490
LLQLFGGTRK
500
DFSHTGRGKF
510
RAEINILLCG
520
DPGTSKSQLL
530
QYVYNLVPRG
540
QYTSGKGSSA
550
VGLTAYVMKD
560
PETRQLVLQT
570
GALVLSDNGI
580
CCIDEFDKMN
590
ESTRSVLHEV
600
MEQQTLSIAK
610
AGIICQLNAR
620
TSVLAAANPI
630
ESQWNPKKTT
640
IENIQLPHTL
650
LSRFDLIFLL
660
LDPQDEAYDR
670
RLAHHLVALY
680
YQSEEQAEEE
690
LLDMAVLKDY
700
IAYAHSTIMP
710
RLSEEASQAL
720
IEAYVDMRKI
730
GSSRGMVSAY
740
PRQLESLIRL
750
AEAHAKVRLS
760
NKVEAIDVEE
770
AKRLHREALK
780
QSATDPRTGI
790
VDISILTTGM
800
SATSRKRKEE
810
LAEALKKLIL
820
SKGKTPALKY
830
QQLFEDIRGQ
840
SDIAITKDMF
850
EEALRALADD
860
DFLTVTGKTV
RLL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000781 |
chromosome, telomeric region |
| Cellular Component |
GO:0071162 |
CMG complex |
| Cellular Component |
GO:0042555 |
MCM complex |
| Cellular Component |
GO:0016020 |
membrane |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0016887 |
ATP hydrolysis activity |
| Molecular Function |
GO:0003678 |
DNA helicase activity |
| Molecular Function |
GO:0003697 |
single-stranded DNA binding |
| Biological Process |
GO:0006260 |
DNA replication |
| Biological Process |
GO:0006271 |
DNA strand elongation involved in DNA replication |
| Biological Process |
GO:0000727 |
double-strand break repair via break-induced replication |
| Biological Process |
GO:1902975 |
mitotic DNA replication initiation |
| Biological Process |
GO:0030174 |
regulation of DNA-templated DNA replication initiation |
Reference
PMID: N/A