Overview
| Uniprot ID | P33992 |
|---|
| Protein Name | DNA replication licensing factor MCM5 |
|---|
| Gene Name | MCM5 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 273 |
MGIYSIKKFGLTTSR |
Function
Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells (PubMed:40940420). Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:16899510, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425)
Protein Sequence
10
MSGFDDPGIF
20
YSDSFGGDAQ
30
ADEGQARKSQ
40
LQRRFKEFLR
50
QYRVGTDRTG
60
FTFKYRDELK
70
RHYNLGEYWI
80
EVEMEDLASF
90
DEDLADYLYK
100
QPAEHLQLLE
110
EAAKEVADEV
120
TRPRPSGEEV
130
LQDIQVMLKS
140
DASPSSIRSL
150
KSDMMSHLVK
160
IPGIIIAASA
170
VRAKATRISI
180
QCRSCRNTLT
190
NIAMRPGLEG
200
YALPRKCNTD
210
QAGRPKCPLD
220
PYFIMPDKCK
230
CVDFQTLKLQ
240
ELPDAVPHGE
250
MPRHMQLYCD
260
RYLCDKVVPG
270
NRVTIMGIYS
280
IKKFGLTTSR
290
GRDRVGVGIR
300
SSYIRVLGIQ
310
VDTDGSGRSF
320
AGAVSPQEEE
330
EFRRLAALPN
340
VYEVISKSIA
350
PSIFGGTDMK
360
KAIACLLFGG
370
SRKRLPDGLT
380
RRGDINLLML
390
GDPGTAKSQL
400
LKFVEKCSPI
410
GVYTSGKGSS
420
AAGLTASVMR
430
DPSSRNFIME
440
GGAMVLADGG
450
VVCIDEFDKM
460
REDDRVAIHE
470
AMEQQTISIA
480
KAGITTTLNS
490
RCSVLAAANS
500
VFGRWDETKG
510
EDNIDFMPTI
520
LSRFDMIFIV
530
KDEHNEERDV
540
MLAKHVITLH
550
VSALTQTQAV
560
EGEIDLAKLK
570
KFIAYCRVKC
580
GPRLSAEAAE
590
KLKNRYIIMR
600
SGARQHERDS
610
DRRSSIPITV
620
RQLEAIVRIA
630
EALSKMKLQP
640
FATEADVEEA
650
LRLFQVSTLD
660
AALSGTLSGV
670
EGFTSQEDQE
680
MLSRIEKQLK
690
RRFAIGSQVS
700
EHSIIKDFTK
710
QKYPEHAIHK
720
VLQLMLRRGE
730
IQHRMQRKVL
YRLK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000781 |
chromosome, telomeric region |
| Cellular Component |
GO:0071162 |
CMG complex |
| Cellular Component |
GO:0042555 |
MCM complex |
| Cellular Component |
GO:0016020 |
membrane |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0016887 |
ATP hydrolysis activity |
| Molecular Function |
GO:0003678 |
DNA helicase activity |
| Molecular Function |
GO:0003688 |
DNA replication origin binding |
| Molecular Function |
GO:0003697 |
single-stranded DNA binding |
| Biological Process |
GO:0006260 |
DNA replication |
| Biological Process |
GO:0006270 |
DNA replication initiation |
| Biological Process |
GO:0000727 |
double-strand break repair via break-induced replication |
| Biological Process |
GO:0030174 |
regulation of DNA-templated DNA replication initiation |
Reference
[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.