Search Results

Overview

Uniprot IDP35269
Protein NameGeneral transcription factor IIF subunit 1
Gene NameGTF2F1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
169 EEWERRNKVLNHFSI
242 KKKAPLAKGGRKKKK
407 TLRAAASKLEQGKRV
421 VSEMPAAKRLRLDTG
435 GPQSLSGKSTPQPPS
444 TPQPPSGKTTPNSGD
83 REEARRKKYGIVLKE

Function

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation

Protein Sequence

10 MAALGPSSQN 20 VTEYVVRVPK 30 NTTKKYNIMA 40 FNAADKVNFA 50 TWNQARLERD 60 LSNKKIYQEE 70 EMPESGAGSE 80 FNRKLREEAR 90 RKKYGIVLKE 100 FRPEDQPWLL 110 RVNGKSGRKF 120 KGIKKGGVTE 130 NTSYYIFTQC 140 PDGAFEAFPV 150 HNWYNFTPLA 160 RHRTLTAEEA 170 EEEWERRNKV 180 LNHFSIMQQR 190 RLKDQDQDED 200 EEEKEKRGRR 210 KASELRIHDL 220 EDDLEMSSDA 230 SDASGEEGGR 240 VPKAKKKAPL 250 AKGGRKKKKK 260 KGSDDEAFED 270 SDDGDFEGQE 280 VDYMSDGSSS 290 SQEEPESKAK 300 APQQEEGPKG 310 VDEQSDSSEE 320 SEEEKPPEED 330 KEEEEEKKAP 340 TPQEKKRRKD 350 SSEESDSSEE 360 SDIDSEASSA 370 LFMAKKKTPP 380 KRERKPSGGS 390 SRGNSRPGTP 400 SAEGGSTSST 410 LRAAASKLEQ 420 GKRVSEMPAA 430 KRLRLDTGPQ 440 SLSGKSTPQP 450 PSGKTTPNSG 460 DVQVTEDAVR 470 RYLTRKPMTT 480 KDLLKKFQTK 490 KTGLSSEQTV 500 NVLAQILKRL 510 NPERKMINDK MHFSLKE

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005654 nucleoplasm
Molecular Function GO:0019211 phosphatase activator activity
Molecular Function GO:1990841 promoter-specific chromatin binding
Molecular Function GO:0019904 protein domain specific binding
Molecular Function GO:0019903 protein phosphatase binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0016251 RNA polymerase II general transcription initiation factor activity
Molecular Function GO:0001091 RNA polymerase II general transcription initiation factor binding
Molecular Function GO:0001096 TFIIF-class transcription factor complex binding
Biological Process GO:0032091 negative regulation of protein binding
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0032968 positive regulation of transcription elongation by RNA polymerase II
Biological Process GO:0009615 response to virus
Biological Process GO:0006366 transcription by RNA polymerase II
Biological Process GO:0006368 transcription elongation by RNA polymerase II
Biological Process GO:0006367 transcription initiation at RNA polymerase II promoter
Cellular Component GO:0005634 nucleus
Cellular Component GO:0032991 protein-containing complex
Cellular Component GO:0005669 transcription factor TFIID complex
Cellular Component GO:0005674 transcription factor TFIIF complex
Molecular Function GO:0003677 DNA binding

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[5] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[6] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[7] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[8] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[9] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[10] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.