Overview
| Uniprot ID | P35427 |
|---|
| Protein Name | Large ribosomal subunit protein uL13 |
|---|
| Gene Name | Rpl13a |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 114 |
GIPPPYDKKKRMVVP |
| 125 |
MVVPAALKVVRLKPT |
| 134 |
VRLKPTRKFAYLGRL |
| 148 |
LAHEVGWKYQAVTAT |
| 159 |
VTATLEEKRKEKAKI |
| 191 |
NVEKKICKFTEVLKT |
| 25 |
RLAAIVAKQVLLGRK |
| 91 |
VRGMLPHKTKRGQAA |
Function
Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex. Involved in methylation of rRNA
Protein Sequence
10
MAEGQVLVLD
20
GRSHLLGRLA
30
AIVAKQVLLG
40
RKVVVVRCEG
50
INISGNFYRN
60
KLKYLAFLRK
70
RMNTNPSRGP
80
YHFRAPSRIF
90
WRTVRGMLPH
100
KTKRGQAALE
110
RLKVLDGIPP
120
PYDKKKRMVV
130
PAALKVVRLK
140
PTRKFAYLGR
150
LAHEVGWKYQ
160
AVTATLEEKR
170
KEKAKIHYRK
180
KKQLLRLRKQ
190
AEKNVEKKIC
200
KFTEVLKTNG
LLV
Gene Ontology
| Classification |
GO ID |
Description |
| Biological Process |
GO:0071320 |
cellular response to cAMP |
| Biological Process |
GO:0071480 |
cellular response to gamma radiation |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0022625 |
cytosolic large ribosomal subunit |
| Cellular Component |
GO:0022626 |
cytosolic ribosome |
| Cellular Component |
GO:0097452 |
GAIT complex |
| Cellular Component |
GO:1990904 |
ribonucleoprotein complex |
| Cellular Component |
GO:0005840 |
ribosome |
| Cellular Component |
GO:0045202 |
synapse |
| Molecular Function |
GO:0003729 |
mRNA binding |
| Molecular Function |
GO:0003735 |
structural constituent of ribosome |
| Biological Process |
GO:0071346 |
cellular response to type II interferon |
| Biological Process |
GO:0071493 |
cellular response to UV-B |
| Biological Process |
GO:0002181 |
cytoplasmic translation |
| Biological Process |
GO:0042592 |
homeostatic process |
| Biological Process |
GO:0060425 |
lung morphogenesis |
| Biological Process |
GO:0048246 |
macrophage chemotaxis |
| Biological Process |
GO:1901194 |
negative regulation of formation of translation preinitiation complex |
| Biological Process |
GO:0017148 |
negative regulation of translation |
| Biological Process |
GO:0032496 |
response to lipopolysaccharide |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.