Search Results

Overview

Uniprot IDP35579
Protein NameMyosin-9
Gene NameMYH9
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1014 NLTEEEEKSKSLAKL
1016 TEEEEKSKSLAKLKN
1024 SLAKLKNKHEAMITD
1081 LKMQLAKKEEELQAA
1099 VEEEAAQKNMALKKI
1173 EQEVNILKKTLEEEA
1174 QEVNILKKTLEEEAK
1193 QIQEMRQKHSQAVEE
1209 AEQLEQTKRVKANLE
1212 LEQTKRVKANLEKAK
1219 KANLEKAKQTLENER
1234 GELANEVKVLLQGKG
1240 VKVLLQGKGDSEHKR
1249 DSEHKRKKVEAQLQE
1274 VRTELADKVTKLQVE
1324 LQEENRQKLSLSTKL
1332 LSLSTKLKQVEDEKN
1338 LKQVEDEKNSFREQL
1352 LEEEEEAKHNLEKQI
1357 EAKHNLEKQIATLHA
1370 HAQVADMKKKMEDSV
1371 AQVADMKKKMEDSVG
1372 QVADMKKKMEDSVGC
1392 EVKRKLQKDLEGLSQ
1404 LSQRHEEKVAAYDKL
1410 EKVAAYDKLEKTKTR
1441 QSACNLEKKQKKFDQ
1445 NLEKKQKKFDQLLAE
1454 DQLLAEEKTISAKYA
1459 EEKTISAKYAEERDR
1477 EAREKETKALSLARA
1492 LEEAMEQKAELERLN
1518 SSKDDVGKSVHELEK
1525 KSVHELEKSKRALEQ
1603 AELEDERKQRSMAVA
1614 MAVAARKKLEMDLKD
1638 KNRDEAIKQLRKLQA
1642 EAIKQLRKLQAQMKD
1669 EEILAQAKENEKKLK
1724 GALALEEKRRLEARI
1775 LERSHAQKNENARQQ
1793 QNKELKVKLQEMEGT
1802 QEMEGTVKSKYKASI
1806 GTVKSKYKASITALE
1828 EQLDNETKERQAACK
1835 KERQAACKQVRRTEK
1845 RRTEKKLKDVLLQVD
1862 RRNAEQYKDQADKAS
1918 NREVSSLKNKLRRGD
1920 EVSSLKNKLRRGDLP
199 AYVASSHKSKKDQGE
201 VASSHKSKKDQGELE
202 ASSHKSKKDQGELER
228 FGNAKTVKNDNSSRF
29 AQADWAAKKLVWVPS
299 LLLEPYNKYRFLSNG
30 QADWAAKKLVWVPSD
403 VGRDYVQKAQTKEQA
435 WLVLRINKALDKTKR
540 WFPKATDKSFVEKVM
555 QEQGTHPKFQKPKQL
560 HPKFQKPKQLKDKAD
565 KPKQLKDKADFCIIH
613 KFVSELWKDVDRIIG
651 RTVGQLYKEQLAKLM
678 CIIPNHEKKAGKLDP
74 VNKDDIQKMNPPKFS
760 LYRIGQSKVFFRAGV
79 IQKMNPPKFSKVEDM
8 MAQQAADKYLYVDKN
821 RNCAAYLKLRNWQWW
835 WRLFTKVKPLLQVSR
856 AKEEELVKVREKQLA
860 ELVKVREKQLAAENR
910 RARLTAKKQELEEIC
961 EEESARQKLQLEKVT
966 RQKLQLEKVTTEAKL
972 EKVTTEAKLKKLEEE
974 VTTEAKLKKLEEEQI
975 TTEAKLKKLEEEQII

Function

Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Required for cortical actin clearance prior to oocyte exocytosis (By similarity). Promotes cell motility in conjunction with S100A4 (PubMed:16707441). During cell spreading, plays an important role in cytoskeleton reorganization, focal contact formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10 (PubMed:20052411)

Protein Sequence

10 MAQQAADKYL 20 YVDKNFINNP 30 LAQADWAAKK 40 LVWVPSDKSG 50 FEPASLKEEV 60 GEEAIVELVE 70 NGKKVKVNKD 80 DIQKMNPPKF 90 SKVEDMAELT 100 CLNEASVLHN 110 LKERYYSGLI 120 YTYSGLFCVV 130 INPYKNLPIY 140 SEEIVEMYKG 150 KKRHEMPPHI 160 YAITDTAYRS 170 MMQDREDQSI 180 LCTGESGAGK 190 TENTKKVIQY 200 LAYVASSHKS 210 KKDQGELERQ 220 LLQANPILEA 230 FGNAKTVKND 240 NSSRFGKFIR 250 INFDVNGYIV 260 GANIETYLLE 270 KSRAIRQAKE 280 ERTFHIFYYL 290 LSGAGEHLKT 300 DLLLEPYNKY 310 RFLSNGHVTI 320 PGQQDKDMFQ 330 ETMEAMRIMG 340 IPEEEQMGLL 350 RVISGVLQLG 360 NIVFKKERNT 370 DQASMPDNTA 380 AQKVSHLLGI 390 NVTDFTRGIL 400 TPRIKVGRDY 410 VQKAQTKEQA 420 DFAIEALAKA 430 TYERMFRWLV 440 LRINKALDKT 450 KRQGASFIGI 460 LDIAGFEIFD 470 LNSFEQLCIN 480 YTNEKLQQLF 490 NHTMFILEQE 500 EYQREGIEWN 510 FIDFGLDLQP 520 CIDLIEKPAG 530 PPGILALLDE 540 ECWFPKATDK 550 SFVEKVMQEQ 560 GTHPKFQKPK 570 QLKDKADFCI 580 IHYAGKVDYK 590 ADEWLMKNMD 600 PLNDNIATLL 610 HQSSDKFVSE 620 LWKDVDRIIG 630 LDQVAGMSET 640 ALPGAFKTRK 650 GMFRTVGQLY 660 KEQLAKLMAT 670 LRNTNPNFVR 680 CIIPNHEKKA 690 GKLDPHLVLD 700 QLRCNGVLEG 710 IRICRQGFPN 720 RVVFQEFRQR 730 YEILTPNSIP 740 KGFMDGKQAC 750 VLMIKALELD 760 SNLYRIGQSK 770 VFFRAGVLAH 780 LEEERDLKIT 790 DVIIGFQACC 800 RGYLARKAFA 810 KRQQQLTAMK 820 VLQRNCAAYL 830 KLRNWQWWRL 840 FTKVKPLLQV 850 SRQEEEMMAK 860 EEELVKVREK 870 QLAAENRLTE 880 METLQSQLMA 890 EKLQLQEQLQ 900 AETELCAEAE 910 ELRARLTAKK 920 QELEEICHDL 930 EARVEEEEER 940 CQHLQAEKKK 950 MQQNIQELEE 960 QLEEEESARQ 970 KLQLEKVTTE 980 AKLKKLEEEQ 990 IILEDQNCKL 1000 AKEKKLLEDR 1010 IAEFTTNLTE 1020 EEEKSKSLAK 1030 LKNKHEAMIT 1040 DLEERLRREE 1050 KQRQELEKTR 1060 RKLEGDSTDL 1070 SDQIAELQAQ 1080 IAELKMQLAK 1090 KEEELQAALA 1100 RVEEEAAQKN 1110 MALKKIRELE 1120 SQISELQEDL 1130 ESERASRNKA 1140 EKQKRDLGEE 1150 LEALKTELED 1160 TLDSTAAQQE 1170 LRSKREQEVN 1180 ILKKTLEEEA 1190 KTHEAQIQEM 1200 RQKHSQAVEE 1210 LAEQLEQTKR 1220 VKANLEKAKQ 1230 TLENERGELA 1240 NEVKVLLQGK 1250 GDSEHKRKKV 1260 EAQLQELQVK 1270 FNEGERVRTE 1280 LADKVTKLQV 1290 ELDNVTGLLS 1300 QSDSKSSKLT 1310 KDFSALESQL 1320 QDTQELLQEE 1330 NRQKLSLSTK 1340 LKQVEDEKNS 1350 FREQLEEEEE 1360 AKHNLEKQIA 1370 TLHAQVADMK 1380 KKMEDSVGCL 1390 ETAEEVKRKL 1400 QKDLEGLSQR 1410 HEEKVAAYDK 1420 LEKTKTRLQQ 1430 ELDDLLVDLD 1440 HQRQSACNLE 1450 KKQKKFDQLL 1460 AEEKTISAKY 1470 AEERDRAEAE 1480 AREKETKALS 1490 LARALEEAME 1500 QKAELERLNK 1510 QFRTEMEDLM 1520 SSKDDVGKSV 1530 HELEKSKRAL 1540 EQQVEEMKTQ 1550 LEELEDELQA 1560 TEDAKLRLEV 1570 NLQAMKAQFE 1580 RDLQGRDEQS 1590 EEKKKQLVRQ 1600 VREMEAELED 1610 ERKQRSMAVA 1620 ARKKLEMDLK 1630 DLEAHIDSAN 1640 KNRDEAIKQL 1650 RKLQAQMKDC 1660 MRELDDTRAS 1670 REEILAQAKE 1680 NEKKLKSMEA 1690 EMIQLQEELA 1700 AAERAKRQAQ 1710 QERDELADEI 1720 ANSSGKGALA 1730 LEEKRRLEAR 1740 IAQLEEELEE 1750 EQGNTELIND 1760 RLKKANLQID 1770 QINTDLNLER 1780 SHAQKNENAR 1790 QQLERQNKEL 1800 KVKLQEMEGT 1810 VKSKYKASIT 1820 ALEAKIAQLE 1830 EQLDNETKER 1840 QAACKQVRRT 1850 EKKLKDVLLQ 1860 VDDERRNAEQ 1870 YKDQADKAST 1880 RLKQLKRQLE 1890 EAEEEAQRAN 1900 ASRRKLQREL 1910 EDATETADAM 1920 NREVSSLKNK 1930 LRRGDLPFVV 1940 PRRMARKGAG 1950 DGSDEEVDGK 1960 ADGAEAKPAE

Gene Ontology

Classification GO ID Description
Cellular Component GO:0015629 actin cytoskeleton
Cellular Component GO:0042641 actomyosin
Cellular Component GO:0005826 actomyosin contractile ring
Cellular Component GO:0005912 adherens junction
Cellular Component GO:0005903 brush border
Cellular Component GO:0031252 cell leading edge
Cellular Component GO:0009986 cell surface
Cellular Component GO:0032154 cleavage furrow
Cellular Component GO:0060473 cortical granule
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0009898 cytoplasmic side of plasma membrane
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005925 focal adhesion
Cellular Component GO:0005794 Golgi apparatus
Cellular Component GO:0001772 immunological synapse
Cellular Component GO:0016020 membrane
Cellular Component GO:0032982 myosin filament
Cellular Component GO:0016460 myosin II complex
Cellular Component GO:0097513 myosin II filament
Cellular Component GO:0031594 neuromuscular junction
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0032991 protein-containing complex
Cellular Component GO:0001726 ruffle
Cellular Component GO:0005819 spindle
Cellular Component GO:0001725 stress fiber
Cellular Component GO:0001931 uropod
Molecular Function GO:0003779 actin binding
Molecular Function GO:0051015 actin filament binding
Molecular Function GO:0043531 ADP binding
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0045296 cadherin binding
Molecular Function GO:0005516 calmodulin binding
Molecular Function GO:0003774 cytoskeletal motor activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0005178 integrin binding
Molecular Function GO:0000146 microfilament motor activity
Molecular Function GO:0019904 protein domain specific binding
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0043495 protein-membrane adaptor activity
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0001618 virus receptor activity
Biological Process GO:0030036 actin cytoskeleton organization
Biological Process GO:0030048 actin filament-based movement
Biological Process GO:0031032 actomyosin structure organization
Biological Process GO:0001525 angiogenesis
Biological Process GO:0043534 blood vessel endothelial cell migration
Biological Process GO:0060471 cortical granule exocytosis
Biological Process GO:0032506 cytokinetic process
Biological Process GO:0007229 integrin-mediated signaling pathway
Biological Process GO:0050900 leukocyte migration
Biological Process GO:0032418 lysosome localization
Biological Process GO:0006509 membrane protein ectodomain proteolysis
Biological Process GO:0030224 monocyte differentiation
Biological Process GO:1903919 negative regulation of actin filament severing
Biological Process GO:0006911 phagocytosis, engulfment
Biological Process GO:0001778 plasma membrane repair
Biological Process GO:0070527 platelet aggregation
Biological Process GO:0030220 platelet formation
Biological Process GO:1903923 positive regulation of protein processing in phagocytic vesicle
Biological Process GO:0015031 protein transport
Biological Process GO:0045055 regulated exocytosis
Biological Process GO:0008360 regulation of cell shape
Biological Process GO:1905684 regulation of plasma membrane repair
Biological Process GO:0046718 symbiont entry into host cell

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[5] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[6] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[7] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[8] Yan M, Tu H, Tang S, Gai Z, Shi Q et al.. Lactylated Proteomic Analysis Reveals Functional Implications of Lysine Lactylation In Asthenozoospermia.. Mol Cell Proteomics 24(12):101439. 2025 Dec. PMID: 41192556.

[9] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.