Overview
| Uniprot ID | P38606 |
|---|
| Protein Name | V-type proton ATPase catalytic subunit A |
|---|
| Gene Name | ATP6V1A |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 139 |
KWDFTPCKNLRVGSH |
| 163 |
VSENSLIKHKIMLPP |
| 220 |
QVRPVTEKLPANHPL |
| 467 |
ALDEYYDKHFTEFVP |
| 5 |
***MDFSKLPKILDE |
| 513 |
KITLEVAKLIKDDFL |
Function
Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:8463241, PubMed:22053050). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it promotes acidification of the extracellular environment (PubMed:32001091). The V-ATPase complex also acts as an activator for mTORC1 on lysosomal membrane by promoting the guanine nucleotide exchange factor (GEF) of the Ragulator complex, thereby enabling mTORC1 recruitment (PubMed:22053050). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633). May play a role in neurite development and synaptic connectivity (PubMed:29668857)
Protein Sequence
10
MDFSKLPKIL
20
DEDKESTFGY
30
VHGVSGPVVT
40
ACDMAGAAMY
50
ELVRVGHSEL
60
VGEIIRLEGD
70
MATIQVYEET
80
SGVSVGDPVL
90
RTGKPLSVEL
100
GPGIMGAIFD
110
GIQRPLSDIS
120
SQTQSIYIPR
130
GVNVSALSRD
140
IKWDFTPCKN
150
LRVGSHITGG
160
DIYGIVSENS
170
LIKHKIMLPP
180
RNRGTVTYIA
190
PPGNYDTSDV
200
VLELEFEGVK
210
EKFTMVQVWP
220
VRQVRPVTEK
230
LPANHPLLTG
240
QRVLDALFPC
250
VQGGTTAIPG
260
AFGCGKTVIS
270
QSLSKYSNSD
280
VIIYVGCGER
290
GNEMSEVLRD
300
FPELTMEVDG
310
KVESIMKRTA
320
LVANTSNMPV
330
AAREASIYTG
340
ITLSEYFRDM
350
GYHVSMMADS
360
TSRWAEALRE
370
ISGRLAEMPA
380
DSGYPAYLGA
390
RLASFYERAG
400
RVKCLGNPER
410
EGSVSIVGAV
420
SPPGGDFSDP
430
VTSATLGIVQ
440
VFWGLDKKLA
450
QRKHFPSVNW
460
LISYSKYMRA
470
LDEYYDKHFT
480
EFVPLRTKAK
490
EILQEEEDLA
500
EIVQLVGKAS
510
LAETDKITLE
520
VAKLIKDDFL
530
QQNGYTPYDR
540
FCPFYKTVGM
550
LSNMIAFYDM
560
ARRAVETTAQ
570
SDNKITWSII
580
REHMGDILYK
590
LSSMKFKDPL
600
KDGEAKIKSD
610
YAQLLEDMQN
AFRSLED
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0016324 |
apical plasma membrane |
| Cellular Component |
GO:0030665 |
clathrin-coated vesicle membrane |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0010008 |
endosome membrane |
| Cellular Component |
GO:0070062 |
extracellular exosome |
| Cellular Component |
GO:0098850 |
extrinsic component of synaptic vesicle membrane |
| Cellular Component |
GO:0000139 |
Golgi membrane |
| Cellular Component |
GO:0005765 |
lysosomal membrane |
| Cellular Component |
GO:0046612 |
lysosomal proton-transporting V-type ATPase, V1 domain |
| Cellular Component |
GO:0016020 |
membrane |
| Cellular Component |
GO:0005902 |
microvillus |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0016469 |
proton-transporting two-sector ATPase complex |
| Cellular Component |
GO:0030141 |
secretory granule |
| Cellular Component |
GO:0000221 |
vacuolar proton-transporting V-type ATPase, V1 domain |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0016887 |
ATP hydrolysis activity |
| Molecular Function |
GO:0046961 |
proton-transporting ATPase activity, rotational mechanism |
| Biological Process |
GO:0046034 |
ATP metabolic process |
| Biological Process |
GO:0036295 |
cellular response to increased oxygen levels |
| Biological Process |
GO:0048388 |
endosomal lumen acidification |
| Biological Process |
GO:0061795 |
Golgi lumen acidification |
| Biological Process |
GO:0006879 |
intracellular iron ion homeostasis |
| Biological Process |
GO:0051452 |
intracellular pH reduction |
| Biological Process |
GO:0007042 |
lysosomal lumen acidification |
| Biological Process |
GO:1902600 |
proton transmembrane transport |
| Biological Process |
GO:0016241 |
regulation of macroautophagy |
| Biological Process |
GO:0097401 |
synaptic vesicle lumen acidification |
| Biological Process |
GO:0007035 |
vacuolar acidification |
Reference
[1] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.