Search Results

Overview

Uniprot ID	P39052
Protein Name	Dynamin-2
Gene Name	Dnm2
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
393	REISYAIKNIHGVRT

Function

Catalyzes the hydrolysis of GTP and utilizes this energy to mediate vesicle scission at plasma membrane during endocytosis and filament remodeling at many actin structures during organization of the actin cytoskeleton (PubMed:19605363, PubMed:19995918, PubMed:35620056). Plays an important role in vesicular trafficking processes, namely clathrin-mediated endocytosis (CME), exocytic and clathrin-coated vesicle from the trans-Golgi network, and PDGF stimulated macropinocytosis (PubMed:19995918, PubMed:21195118, PubMed:9438853). During vesicular trafficking process, associates to the membrane, through lipid binding, and self-assembles into ring-like structure through oligomerization to form a helical polymer around the vesicle membrane and leading to vesicle scission (PubMed:35620056). Plays a role in organization of the actin cytoskeleton by mediating arrangement of stress fibers and actin bundles in podocytes (PubMed:35620056). During organization of the actin cytoskeleton, self-assembles into ring-like structure that directly bundles actin filaments to form typical membrane tubules decorated with dynamin spiral polymers (PubMed:35620056). Self-assembly increases GTPase activity and the GTP hydrolysis causes the rapid depolymerization of dynamin spiral polymers, and results in dispersion of actin bundles (PubMed:35620056). Remodels, through its interaction with CTTN, bundled actin filaments in a GTPase-dependent manner and plays a role in orchestrating the global actomyosin cytoskeleton (PubMed:19605363). The interaction with CTTN stabilizes the interaction of DNM2 and actin filaments and stimulates the intrinsic GTPase activity that results in actin filament-barbed ends and increases the sensitivity of filaments in bundles to the actin depolymerizing factor, CFL1 (PubMed:19605363). Plays a role in the autophagy process, by participating in the formation of ATG9A vesicles destined for the autophagosomes through its interaction with SNX18, by mediating recycling endosome scission leading to autophagosome release through MAP1LC3B interaction and by regulating maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane (By similarity). Also plays a role in cytokinesis (PubMed:21195118). May participate in centrosome cohesion through its interaction with TUBG1 (PubMed:15048127). Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (PubMed:21210813). Involved in membrane tubulation (By similarity)

Protein Sequence

10 MGNRGMEELI 20 PLVNKLQDAF 30 SSIGQSCHLD 40 LPQIAVVGGQ 50 SAGKSSVLEN 60 FVGRDFLPRG 70 SGIVTRRPLI 80 LQLIFSKTEY 90 AEFLHCKSKK 100 FTDFDEVRQE 110 IEAETDRVTG 120 TNKGISPVPI 130 NLRVYSPHVL 140 NLTLIDLPGI 150 TKVPVGDQPP 160 DIEYQIKDMI 170 LQFISRESSL 180 ILAVTPANMD 190 LANSDALKLA 200 KEVDPQGLRT 210 IGVITKLDLM 220 DEGTDARDVL 230 ENKLLPLRRG 240 YIGVVNRSQK 250 DIEGRKDIRA 260 ALAAERKFFL 270 SHPAYRHMAD 280 RMGTPHLQKT 290 LNQQLTNHIR 300 ESLPTLRSKL 310 QSQLLSLEKE 320 VEEYKNFRPD 330 DPTRKTKALL 340 QMVQQFGVDF 350 EKRIEGSGDQ 360 VDTLELSGGA 370 RINRIFHERF 380 PFELVKMEFD 390 EKDLRREISY 400 AIKNIHGVRT 410 GLFTPDLAFE 420 AIVKKQVVKL 430 KEPCLKCVDL 440 VIQELISTVR 450 QCTSKLSSYP 460 RLREETERIV 470 TTYIREREGR 480 TKDQILLLID 490 IEQSYINTNH 500 EDFIGFANAQ 510 QRSTQLNKKR 520 AIPNQGEILV 530 IRRGWLTINN 540 ISLMKGGSKE 550 YWFVLTAESL 560 SWYKDEEEKE 570 KKYMLPLDNL 580 KIRDVEKGFM 590 SNKHVFAIFN 600 TEQRNVYKDL 610 RQIELACDSQ 620 EDVDSWKASF 630 LRAGVYPEKD 640 QAENEDGAQE 650 NTFSMDPQLE 660 RQVETIRNLV 670 DSYVAIINKS 680 IRDLMPKTIM 690 HLMINNTKAF 700 IHHELLAYLY 710 SSADQSSLME 720 ESAEQAQRRD 730 DMLRMYHALK 740 EALNIIGDIS 750 TSTVSTPVPP 760 PVDDTWLQNT 770 SSHSPTPQRR 780 PVSSVHPPGR 790 PPAVRGPTPG 800 PPLIPMPVGA 810 TSSFSAPPIP 820 SRPGPQNVFA 830 NNDPFSAPPQ 840 IPSRPARIPP 850 GIPPGVPSRR 860 APAAPSRPTI 870 IRPAEPSLLD

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0070161	anchoring junction
Biological Process	GO:0006898	receptor-mediated endocytosis
Biological Process	GO:0030516	regulation of axon extension
Biological Process	GO:1903358	regulation of Golgi organization
Biological Process	GO:0035020	regulation of Rac protein signal transduction
Biological Process	GO:0042220	response to cocaine
Biological Process	GO:0009416	response to light stimulus
Biological Process	GO:0007283	spermatogenesis
Biological Process	GO:0043149	stress fiber assembly
Biological Process	GO:0016185	synaptic vesicle budding from presynaptic endocytic zone membrane
Biological Process	GO:0048488	synaptic vesicle endocytosis
Biological Process	GO:0033572	transferrin transport
Biological Process	GO:0003281	ventricular septum development
Biological Process	GO:0099050	vesicle scission
Cellular Component	GO:0005938	cell cortex
Cellular Component	GO:0030054	cell junction
Cellular Component	GO:0005814	centriole
Cellular Component	GO:0005813	centrosome
Cellular Component	GO:0045334	clathrin-coated endocytic vesicle
Cellular Component	GO:0005905	clathrin-coated pit
Cellular Component	GO:0030136	clathrin-coated vesicle
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005768	endosome
Cellular Component	GO:0098978	glutamatergic synapse
Cellular Component	GO:0000139	Golgi membrane
Cellular Component	GO:0030027	lamellipodium
Cellular Component	GO:0072687	meiotic spindle
Cellular Component	GO:0005874	microtubule
Cellular Component	GO:0030496	midbody
Cellular Component	GO:0043005	neuron projection
Cellular Component	GO:0048471	perinuclear region of cytoplasm
Cellular Component	GO:0001891	phagocytic cup
Cellular Component	GO:0030670	phagocytic vesicle membrane
Cellular Component	GO:0001917	photoreceptor inner segment
Cellular Component	GO:0005886	plasma membrane
Cellular Component	GO:0002102	podosome
Cellular Component	GO:0014069	postsynaptic density
Cellular Component	GO:0099092	postsynaptic density, intracellular component
Cellular Component	GO:0098843	postsynaptic endocytic zone
Cellular Component	GO:0045211	postsynaptic membrane
Cellular Component	GO:0098793	presynapse
Cellular Component	GO:0032991	protein-containing complex
Cellular Component	GO:0055037	recycling endosome
Cellular Component	GO:0032587	ruffle membrane
Cellular Component	GO:0045202	synapse
Cellular Component	GO:0005802	trans-Golgi network
Cellular Component	GO:0001931	uropod
Molecular Function	GO:0031749	D2 dopamine receptor binding
Molecular Function	GO:0005525	GTP binding
Molecular Function	GO:0003924	GTPase activity
Molecular Function	GO:0008017	microtubule binding
Molecular Function	GO:0050998	nitric-oxide synthase binding
Molecular Function	GO:0036312	phosphatidylinositol 3-kinase regulatory subunit binding
Molecular Function	GO:0005546	phosphatidylinositol-4,5-bisphosphate binding
Molecular Function	GO:0019901	protein kinase binding
Molecular Function	GO:0120283	protein serine/threonine kinase binding
Molecular Function	GO:0044877	protein-containing complex binding
Molecular Function	GO:0017124	SH3 domain binding
Molecular Function	GO:0050699	WW domain binding
Biological Process	GO:0061572	actin filament bundle organization
Biological Process	GO:0035904	aorta development
Biological Process	GO:0006914	autophagy
Biological Process	GO:0071245	cellular response to carbon monoxide
Biological Process	GO:1903351	cellular response to dopamine
Biological Process	GO:0071732	cellular response to nitric oxide
Biological Process	GO:0071481	cellular response to X-ray
Biological Process	GO:0007098	centrosome cycle
Biological Process	GO:0060976	coronary vasculature development
Biological Process	GO:0006897	endocytosis
Biological Process	GO:0002031	G protein-coupled receptor internalization
Biological Process	GO:0006893	Golgi to plasma membrane transport
Biological Process	GO:0044351	macropinocytosis
Biological Process	GO:0097749	membrane tubulation
Biological Process	GO:1903526	negative regulation of membrane tubulation
Biological Process	GO:1902856	negative regulation of non-motile cilium assembly
Biological Process	GO:0030512	negative regulation of transforming growth factor beta receptor signaling pathway
Biological Process	GO:0048812	neuron projection morphogenesis
Biological Process	GO:0006909	phagocytosis
Biological Process	GO:2000370	positive regulation of clathrin-dependent endocytosis
Biological Process	GO:0045807	positive regulation of endocytosis
Biological Process	GO:0010592	positive regulation of lamellipodium assembly
Biological Process	GO:0045429	positive regulation of nitric oxide biosynthetic process
Biological Process	GO:1903408	positive regulation of P-type sodium:potassium-exchanging transporter activity
Biological Process	GO:0050766	positive regulation of phagocytosis
Biological Process	GO:1900026	positive regulation of substrate adhesion-dependent cell spreading
Biological Process	GO:0140239	postsynaptic endocytosis
Biological Process	GO:0051258	protein polymerization
Biological Process	GO:0031623	receptor internalization

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.